Logo des Robert Koch-InstitutLogo des Robert Koch-Institut
Publikationsserver des Robert Koch-Institutsedoc
de|en
Publikation anzeigen 
  • edoc Startseite
  • Artikel in Fachzeitschriften
  • Artikel in Fachzeitschriften
  • Publikation anzeigen
  • edoc Startseite
  • Artikel in Fachzeitschriften
  • Artikel in Fachzeitschriften
  • Publikation anzeigen
JavaScript is disabled for your browser. Some features of this site may not work without it.
Gesamter edoc-ServerBereiche & SammlungenTitelAutorSchlagwortDiese SammlungTitelAutorSchlagwort
PublizierenEinloggenRegistrierenHilfe
StatistikNutzungsstatistik
Gesamter edoc-ServerBereiche & SammlungenTitelAutorSchlagwortDiese SammlungTitelAutorSchlagwort
PublizierenEinloggenRegistrierenHilfe
StatistikNutzungsstatistik
Publikation anzeigen 
  • edoc Startseite
  • Artikel in Fachzeitschriften
  • Artikel in Fachzeitschriften
  • Publikation anzeigen
  • edoc Startseite
  • Artikel in Fachzeitschriften
  • Artikel in Fachzeitschriften
  • Publikation anzeigen
2013-08-08Zeitschriftenartikel DOI: 10.1371/journal.pone.0073287
LPS Structure and PhoQ Activity Are Important for Salmonella Typhimurium Virulence in the Gallleria mellonella Infection Model
Bender, Jennifer K.
Wille, Thorsten
Blank, Kathrin
Lange, Anna
Gerlach, Roman
The larvae of the wax moth, Galleria mellonella, have been used experimentally to host a range of bacterial and fungal pathogens. In this study we evaluated the suitability of G. mellonella as an alternative animal model of Salmonella infection. Using a range of inoculum doses we established that the LD50 of Salmonella Typhimurium strain NCTC 12023 was 3.6 × 103 bacteria per larva. Further, a set of isogenic mutant strains depleted of known virulence factors was tested to identify determinants essential for S. Typhimurium pathogenesis. Mutants depleted of one or both of the type III secretion systems encoded by Salmonella Pathogenicity Islands 1 and 2 showed no virulence defect. In contrast, we observed reduced pathogenic potential of a phoQ mutant indicating an important role for the PhoPQ two-component signal transduction system. Lipopolysaccharide (LPS) structure was also shown to influence Salmonella virulence in G. mellonella. A waaL (rfaL) mutant, which lacks the entire O-antigen (OAg), was virtually avirulent, while a wzzST/wzzfepE double mutant expressing only a very short OAg was highly attenuated for virulence. Furthermore, shortly after infection both LPS mutant strains showed decreased replication when compared to the wild type in a flow cytometry-based competitive index assay. In this study we successfully established a G. mellonella model of S. Typhimurium infection. By identifying PhoQ and LPS OAg length as key determinants of virulence in the wax moth larvae we proved that there is an overlap between this and other animal model systems, thus confirming that the G. mellonella infection model is suitable for assessing aspects of Salmonella virulence function.
Dateien zu dieser Publikation
Thumbnail
27JIjzxW1JonQ.pdf — PDF — 1.211 Mb
MD5: f149a2d1197980fa74aee8c9a6e7afbd
Zitieren
BibTeX
EndNote
RIS
Keine Lizenzangabe
Zur Langanzeige

Verwandte Publikationen

Anzeige der Publikationen mit ähnlichem Titel, Autor, Urheber und Thema.

  • 2015-06-01Zeitschriftenartikel
    Outbreak of Uncommon O4 Non-Agglutinating Salmonella Typhimurium Linked to Minced Pork, Saxony-Anhalt, Germany, January to April 2013 
    Alt, Katja; Simon, Sandra; Helmeke, Carina; Kohlstock, Claudia; Prager, Rita; Tietze, Erhard; Rabsch, Wolfgang; Karagiannis, Ioannis; Werber, Dirk; Frank, Christina; Fruth, Angelika
    Introduction: In January 2013, the National Reference Centre for Salmonella (NRC) detected a salmonellosis cluster in Saxony-Anhalt, Germany, caused by uncommon O4 non-agglutinating, monophasic Salmonella (S.) Typhimurium ...
  • 2014-01-02Zeitschriftenartikel
    Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms 
    Nedialkova, Lubov Petkova; Denzler, Rémy; Koeppel, Martin B.; Diehl, Manuel; Ring, Diana; Wille, Thorsten; Gerlach, Roman; Stecher, Bärbel
    The host's immune system plays a key role in modulating growth of pathogens and the intestinal microbiota in the gut. In particular, inflammatory bowel disorders and pathogen infections induce shifts of the resident commensal ...
  • 2009-12-17Zeitschriftenartikel
    Morphology of Salmonella Typhimurium typing phages of the Lilleengen set 
    Eisenstark, Abraham; Rabsch, Wolfgang; Ackermann, Hans-W.
    The Lilleengen scheme for typing Salmonella enterica serovar Typhimurium consists of 12 tailed phages. Ten phages are podoviruses and morphologically identical to Salmonella phage P22. Two phages are siphoviruses and ...
Nutzungsbedingungen Impressum Leitlinien Datenschutzerklärung Kontakt

Das Robert Koch-Institut ist ein Bundesinstitut im

Geschäftsbereich des Bundesministeriums für Gesundheit

© Robert Koch Institut

Alle Rechte vorbehalten, soweit nicht ausdrücklich anders vermerkt.

 
DOI
10.1371/journal.pone.0073287
Permanent URL
https://doi.org/10.1371/journal.pone.0073287
HTML
<a href="https://doi.org/10.1371/journal.pone.0073287">https://doi.org/10.1371/journal.pone.0073287</a>