2013-09-09Zeitschriftenartikel DOI: 10.1371/journal.pone.0073052
Serological Evidence of Asymptomatic Infections during Escherichia coli O104:H4 Outbreak in Germany in 2011
Lassen, Sofie Gillesberg
Introduction: The largest known outbreak caused by a rare hybrid strain of Shiga toxin-producing E.coli (STEC) and enteroaggregative E. coli (EAEC) (E.coli O104:H4) of serotype O104:H4 occurred in Germany in 2011. Fenugreek sprouts acted as a transmission vehicle and were widely consumed in the outbreak area at the time of the epidemic. In total 3,842 people developed a clinical illness caused by this strain; however the rates of asymptomatic infections remain unclear. We aimed to develop a serological assay for detection of E.coli O104 LPS specific antibodies and to establish the post-outbreak levels of seropositivity among people with documented exposure to contaminated sprouts. Results and Discussion: Developed serological assays (ELISA with 84% sensitivity, 63% specificity and Western Blot with 100% sensitivity, 82.5% specificity) identified 33% (16/49) level of asymptomatic infection. Relatively small sample size and a significant time- lapse between the onset of symptoms and serum samples collection (appr. 8 weeks) might explain the assay variability. No association was found between clinical or demographic characteristics and assay positivity. Larger studies are needed to understand the complexity of human immune response and factors influencing development of clinical symptoms. Development of intra-outbreak research plans will substantially aid the conduct of more thorough scientific investigation during an outbreak period.
Dateien zu dieser Publikation
Anzeige der Publikationen mit ähnlichem Titel, Autor, Urheber und Thema.
2015-07-31ZeitschriftenartikelHighly Virulent Non-O157 Enterohemorrhagic Escherichia coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights into the Evolution of EHEC Eichhorn, Inga; Heidemanns, Katrin; Semmler, Torsten; Kinnemann, Bianca; Mellmann, Alexander; Harmsen, Dag; Anjum, Muna F.; Schmidt, Herbert; Fruth, Angelika; Valentin-Weigand, Peter; Heesemann, Jürgen; Suerbaum, Sebastian; Karch, Helge; Wieler, Lothar H.Enterohemorrhagic Escherichia coli (EHEC) is the causative agent of bloody diarrhea and extraintestinal sequelae in humans, most importantly hemolytic-uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). ...
2008-09-11ZeitschriftenartikelCharacterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep Fröhlicher, Erik; Krause, Gladys; Zweifel, Claudio; Beutin, Lothar; Stephan, RogerBackground: Attaching and effacing Escherichia coli (AEEC) are characterized by their ability to cause attaching-and-effacing (A/E) lesions in the gut mucosa of human and animal hosts leading to diarrhoea. The genetic ...
2009-09-01ZeitschriftenartikelPhylogeny and Disease Association of Shiga toxin-Producing Escherichia coli O91 Mellmann, Alexander; Fruth, Angelika; Friedrich, Alexander W.; Wieler, Lothar H.; Harmsen, Dag; Werber, Dirk; Middendorf, Barbara; Bielaszewska, Martina; Karch, HelgeThe diversity and relatedness of 100 Shiga toxin–producing Escherichia coli O91 isolates from different patients were examined by multilocus sequence typing. We identified 10 specific sequence types (ST) and 4 distinct ...