2013-07-12Zeitschriftenartikel DOI: 10.1128/JB.00754-13
DNA Uptake by the Nosocomial Pathogen Acinetobacter baumannii Occurs during Movement along Wet Surfaces
The emergence of Acinetobacter baumannii as an increasingly multidrug-resistant nosocomial pathogen largely relies on acquisition of resistance genes via horizontal gene transfer. Here, we demonstrate that many clinical isolates of A. baumannii take up DNA while they move along wet surfaces. We show that both motility and DNA uptake are abolished after inactivation of pilT, which putatively encodes the type 4 pilus (T4P) retraction ATPase, and comEC, which putatively encodes the DNA uptake channel, respectively. Inactivation of pilT correlates with an increase in the number and length of pili with an average diameter of 7.2 nm. In the Galleria mellonella infection model, the comEC mutant is significantly attenuated, whereas the pilT mutant is not, dissecting biologically distinct roles of T4P and the DNA uptake channel. Collectively, these findings promote our understanding of the mechanisms of DNA uptake and resistance development in A. baumannii, which may also apply to other important pathogens.
Dateien zu dieser Publikation
Anzeige der Publikationen mit ähnlichem Titel, Autor, Urheber und Thema.
2011-06-21ZeitschriftenartikelMolecular characterization of blaNDM-1 in an Acinetobacter baumannii strain isolated in Germany in 2007 Pfeifer, Yvonne; Wilharm, Gottfried; Zander, Esther; Wichelhaus, Thomas A.; Göttig, Stefan; Hunfeld, Klaus-Peter; Seifert, Harald; Witte, Wolfgang; Higgins, Paul G.Objectives: To investigate the genetic environment of the metallo-β-lactamase gene blaNDM-1 in an Acinetobacter baumannii isolated in 2007 in a German hospital. Methods: Antimicrobial susceptibility testing was performed ...
2014-07-07ZeitschriftenartikelImpact of Acinetobacter baumannii Superoxide Dismutase on Motility, Virulence, Oxidative Stress Resistance and Susceptibility to Antibiotics Heindorf, Magdalena; Kadari, Mahendar; Heider, Christine; Skiebe, Evelyn; Wilharm, GottfriedAcinetobacter baumannii is a Gram-negative bacterium appearing as an opportunistic pathogen in hospital settings. Superoxide dismutase (SOD) contributes to virulence in several pathogenic bacteria by detoxifying reactive ...
2010-01-27ZeitschriftenartikelIncreasing Occurrence of Multidrug-Resistance in Acinetobacter baumannii Isolates From Four German University Hospitals, 2002–2006 Wadl, Maria; Heckenbach, Kirsten; Noll, Ines; Ziesing, S.; Pfister, W.; Beer, J.; Schubert, S.; Eckmanns, TimBackground: Acinetobacter baumannii can cause severe infections, mainly in critically ill inpatients. Treatment is complicated by multidrug-resistance (MDR). In Germany, to date, little is known on the extent of MDR in A. ...