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2014-07-07Zeitschriftenartikel DOI: 10.1371/journal.pone.0101033
Impact of Acinetobacter baumannii Superoxide Dismutase on Motility, Virulence, Oxidative Stress Resistance and Susceptibility to Antibiotics
dc.contributor.authorHeindorf, Magdalena
dc.contributor.authorKadari, Mahendar
dc.contributor.authorHeider, Christine
dc.contributor.authorSkiebe, Evelyn
dc.contributor.authorWilharm, Gottfried
dc.date.accessioned2018-05-07T17:49:22Z
dc.date.available2018-05-07T17:49:22Z
dc.date.created2014-08-07
dc.date.issued2014-07-07none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rew8Sq3D1nDg/PDF/25aQiJ48GRQ.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1926
dc.description.abstractAcinetobacter baumannii is a Gram-negative bacterium appearing as an opportunistic pathogen in hospital settings. Superoxide dismutase (SOD) contributes to virulence in several pathogenic bacteria by detoxifying reactive oxygen species released in the course of host defense reactions. However, the biological role of SODs in A. baumannii has not yet been elucidated. Here, we inactivated in A. baumannii ATCC 17978 gene A1S_2343, encoding a putative SOD of the Fe-Mn type by transposon insertion, resulting in mutant ATCC 17978 sod2343::Km. The mutation was also introduced in two naturally competent A. baumannii isolates by transformation with chromosomal DNA derived from mutant ATCC 17978 sod2343::Km. We demonstrate that inactivation of sod2343 leads to significant motility defects in all three A. baumannii strains. The mutant strains were more susceptible to oxidative stress compared to their parental strains. Susceptibility to colistin and tetracycline was increased in all mutant strains while susceptibility of the mutants to gentamicin, levofloxacin and imipenem was strain-dependent. In the Galleria mellonella infection model the mutant strains were significantly attenuated. In conclusion, sod2343 plays an important role in motility, resistance to oxidative stress, susceptibility to antibiotics and virulence in A. baumannii.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAnimalseng
dc.subjectMutationeng
dc.subjectAcinetobacter baumannii/metabolismeng
dc.subjectAcinetobacter baumannii/pathogenicityeng
dc.subjectAnti-Bacterial Agents/pharmacologyeng
dc.subjectDrug Resistance Bacterialeng
dc.subjectAcinetobacter baumannii/drug effectseng
dc.subjectAcinetobacter baumannii/enzymologyeng
dc.subjectEnzyme Activation/drug effectseng
dc.subjectLepidoptera/microbiologyeng
dc.subjectMovement/drug effectseng
dc.subjectOxidative Stress/drug effectseng
dc.subjectReactive Oxygen Species/metabolismeng
dc.subjectSuperoxide Dismutase/geneticseng
dc.subjectSuperoxide Dismutase/metabolismeng
dc.subject.ddc610 Medizin
dc.titleImpact of Acinetobacter baumannii Superoxide Dismutase on Motility, Virulence, Oxidative Stress Resistance and Susceptibility to Antibiotics
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10037127
dc.identifier.doi10.1371/journal.pone.0101033
dc.identifier.doihttp://dx.doi.org/10.25646/1851
local.edoc.container-titlePLoS ONE
local.edoc.container-textHeindorf M, Kadari M, Heider C, Skiebe E, Wilharm G (2014) Impact of Acinetobacter baumannii Superoxide Dismutase on Motility, Virulence, Oxidative Stress Resistance and Susceptibility to Antibiotics. PLoS ONE 9(7): e101033.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0101033
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume9
local.edoc.container-issue7
local.edoc.container-year2014

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