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2013-07-18Zeitschriftenartikel DOI: 10.1016/j.ijmm.2013.07.003
Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae
dc.contributor.authorBrzuszkiewicz, Elzbieta
dc.contributor.authorSchulz, Tino
dc.contributor.authorRydzewski, Kerstin
dc.contributor.authorDaniel, Rolf
dc.contributor.authorGillmaier, Nadine
dc.contributor.authorDittmann, Christine
dc.contributor.authorHolland, Gudrun
dc.contributor.authorSchunder, Eva
dc.contributor.authorLautner, Monika
dc.contributor.authorEisenreich, Wolfgang
dc.contributor.authorLück, Christian
dc.contributor.authorHeuner, Klaus
dc.date.accessioned2018-05-07T17:49:44Z
dc.date.available2018-05-07T17:49:44Z
dc.date.created2014-08-13
dc.date.issued2013-07-18none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reIHNZM1t6Q2U/PDF/25O58hi5mDPjI.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1928
dc.description.abstractLegionella oakridgensis is able to cause Legionnaires’ disease, but is less virulent compared to L. pneumophila strains and very rarely associated with human disease. L. oakridgensis is the only species of the family legionellae which is able to grow on media without additional cysteine. In contrast to earlier publications, we found that L. oakridgensis is able to multiply in amoebae. We sequenced the genome of L. oakridgensis type strain OR-10 (ATCC 33761). The genome is smaller than the other yet sequenced Legionella genomes and has a higher G + C-content of 40.9%. L. oakridgensis lacks a flagellum and it also lacks all genes of the flagellar regulon except of the alternative sigma-28 factor FliA and the anti-sigma-28 factor FlgM. Genes encoding structural components of type I, type II, type IV Lvh and type IV Dot/Icm, Sec- and Tat-secretion systems could be identified. Only a limited set of Dot/Icm effector proteins have been recognized within the genome sequence of L. oakridgensis. Like in L. pneumophila strains, various proteins with eukaryotic motifs and eukaryote-like proteins were detected. We could demonstrate that the Dot/Icm system is essential for intracellular replication of L. oakridgensis. Furthermore, we identified new putative virulence factors of Legionella.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectL. oakridgensiseng
dc.subjectDot/Icm effectorseng
dc.subjectAcanthamoeba lenticulataeng
dc.subjectVirulenceeng
dc.subjectHuman pathogeneng
dc.subjectGenome sequenceeng
dc.subject.ddc610 Medizin
dc.titleLegionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10037156
dc.identifier.doi10.1016/j.ijmm.2013.07.003
dc.identifier.doihttp://dx.doi.org/10.25646/1853
local.edoc.container-titleInternational Journal of Medical Microbiology
local.edoc.container-textBrzuszkiewicz, E., Schulz, T., Rydzewski, K., Daniel, R., Gillmaier, N., Dittmann, C., Holland, G., Schunder, E., Lautner, M., Eisenreich, W., Lück, C., Heuner, K. Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae (2013) International Journal of Medical Microbiology, 303 (8), pp. 514-528.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S143842211300091X
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume303
local.edoc.container-issue8
local.edoc.container-year2013

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