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2014-09-29Zeitschriftenartikel DOI: 10.1007/s12223-014-0349-8
Detection of OXA-48-type carbapenemase-producing Enterobacteriaceae in diagnostic laboratories can be enhanced by addition of bicarbonates to cultivation media or reaction buffers
dc.contributor.authorStudentova, Vendula
dc.contributor.authorPapagiannitsis, Costas C.
dc.contributor.authorIzdebski, Radoslaw
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorChudackova, Eva
dc.contributor.authorBergerova, Tamara
dc.contributor.authorGniadkowski, Marek
dc.contributor.authorHrabak, Jaroslav
dc.date.accessioned2018-05-07T18:05:00Z
dc.date.available2018-05-07T18:05:00Z
dc.date.created2015-01-28
dc.date.issued2014-09-29none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rejs6xiRudoI/PDF/23WyXVcUtYLLQ.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2010
dc.description.abstractCarbapenemase-mediated resistance to carbapenems in Enterobacteriaceae has become the main challenge in the treatment and prevention of infections recently. The partially unnoticed spread of OXA-48-type carbapenemase producers is usually assigned to low minimum inhibitory concentrations (MICs) of carbapenems that OXA-48-producing isolates often display. Therefore, there is an urgent need of specific and sensitive methods for isolation and detection of OXA-48 producers in clinical microbiology diagnostics. The influence of bicarbonates on carbapenem MICs against carbapenemase-producing Enterobacteriaceae was tested. We also checked whether the addition of bicarbonates to liquid media supplemented with meropenem may facilitate the selective enrichment of various carbapenemase producers in cultures. Furthermore, the sensitivity of carbapenemase confirmation by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) and spectrophotometric hydrolysis assays upon the addition of NH4HCO3 was examined. The addition of NaHCO3 significantly increased MICs of ertapenem and meropenem for OXA-48 producers. Furthermore, liquid media supplemented with NaHCO3 and meropenem were reliable for the selective enrichment of carbapenemase producers. The presence of NH4HCO3 in buffers used in the spectrophotometric and MALDI-TOF MS carbapenemase detection increased the sensitivity of that assay. Our results demonstrate that bicarbonates in media or reaction buffers can enhance the sensitivity of screening methods and diagnostic tests for carbapenemase producers.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectCulture Media/chemistryeng
dc.subjectMicrobial Sensitivity Tests/methodseng
dc.subjectSensitivity and Specificityeng
dc.subjectEnterobacteriaceae/isolation & purificationeng
dc.subjectEnterobacteriaceae Infections/microbiologyeng
dc.subjectBacterial Proteins/analysiseng
dc.subjectBacterial Proteins/secretioneng
dc.subjectBicarbonateseng
dc.subjectBufferseng
dc.subjectEnterobacteriaceae/enzymologyeng
dc.subjectEnterobacteriaceae Infections/diagnosiseng
dc.subjectSpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationeng
dc.subjectbeta-Lactamases/analysiseng
dc.subjectbeta-Lactamases/secretioneng
dc.subject.ddc610 Medizin
dc.titleDetection of OXA-48-type carbapenemase-producing Enterobacteriaceae in diagnostic laboratories can be enhanced by addition of bicarbonates to cultivation media or reaction buffers
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10038576
dc.identifier.doi10.1007/s12223-014-0349-8
dc.identifier.doihttp://dx.doi.org/10.25646/1935
local.edoc.container-titleFolia Microbiologica
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://link.springer.com/article/10.1007%2Fs12223-014-0349-8
local.edoc.container-publisher-nameSpringer
local.edoc.container-volume60
local.edoc.container-issue2
local.edoc.container-year2015

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