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2015-08-07Zeitschriftenartikel DOI: 10.1371/journal.pone.0134312
Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus
dc.contributor.authorEisenberg, Tobias
dc.contributor.authorNicklas, Werner
dc.contributor.authorMauder, Norman
dc.contributor.authorRau, Jörg
dc.contributor.authorContzen, Matthias
dc.contributor.authorSemmler, Torsten
dc.contributor.authorHofmann, Nicola
dc.contributor.authorAledelbi, Khayrieh
dc.contributor.authorEwers, Christa
dc.date.accessioned2018-05-07T18:26:53Z
dc.date.available2018-05-07T18:26:53Z
dc.date.created2015-08-26
dc.date.issued2015-08-07none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re8HysTP1Xngw/PDF/27PGFNNnAJqWw.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2127
dc.description.abstractThe genus Streptobacillus (S.) remained monotypic for almost 90 years until two new species were recently described. The type species, S. moniliformis, is one of the two etiological agents of rat bite fever, an under-diagnosed, worldwide occurring zoonosis. In a polyphasic approach field isolates and reference strains of S. moniliformis, S. hongkongensis, S. felis as well as divergent isolates were characterized by comparison of molecular data (n = 29) and from the majority also by their physiological as well as proteomic properties (n = 22). Based on growth-independent physiological profiling using VITEK2-compact, API ZYM and the Micronaut system fastidious growth-related difficulties could be overcome and streptobacilli could definitively be typed despite generally few differences. While differing in their isolation sites and dates, S. moniliformis isolates were found to possess almost identical spectra in matrix-assisted laser desorption ionization—time of flight mass spectrometry and Fourier transform infrared spectroscopy. Spectroscopic methods facilitated differentiation of S. moniliformis, S. hongkongensis and S. felis as well as one divergent isolate. Sequencing of 16S rRNA gene as well as functional genes groEL, recA and gyrB revealed only little intraspecific variability, but generally proved suitable for interspecies discrimination between all three taxa and two groups of divergent isolates.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectMiceeng
dc.subjectGenotypeeng
dc.subjectPhenotypeeng
dc.subjectPhylogenyeng
dc.subjectRatseng
dc.subjectBase Sequenceeng
dc.subjectChickenseng
dc.subjectBacterial Adhesioneng
dc.subjectDiscriminant Analysiseng
dc.subjectLikelihood Functionseng
dc.subjectStreptobacillus/cytologyeng
dc.subjectStreptobacillus/geneticseng
dc.subjectStreptobacillus/isolation & purificationeng
dc.subject.ddc610 Medizin
dc.titlePhenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10040756
dc.identifier.doi10.1371/journal.pone.0134312
dc.identifier.doihttp://dx.doi.org/10.25646/2052
local.edoc.container-titlePLoS ONE
local.edoc.container-textEisenberg T, Nicklas W, Mauder N, Rau J, Contzen M, Semmler T, et al. (2015) Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus. PLoS ONE 10(8): e0134312.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134312
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume10
local.edoc.container-issue8
local.edoc.container-year2015

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