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2015-11-13Zeitschriftenartikel DOI: 10.1371/journal.pntd.0004218
Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections
dc.contributor.authorRockstroh, Alexandra
dc.contributor.authorBarzon, Luisa
dc.contributor.authorPacenti, Monia
dc.contributor.authorPalù, Giorgio
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorUlbert, Sebastian
dc.date.accessioned2018-05-07T18:35:29Z
dc.date.available2018-05-07T18:35:29Z
dc.date.created2015-11-20
dc.date.issued2015-11-13none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reZX296m8mBA/PDF/20l2MnqQrVsA6.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2173
dc.description.abstractDengue virus (DENV) is a mosquito-borne flavivirus and a major international public health concern in many tropical and sub-tropical areas worldwide. DENV is divided into four major serotypes, and infection with one serotype leads to immunity against the same, but not the other serotypes. The specific diagnosis of DENV-infections via antibody-detection is problematic due to the high degree of cross-reactivity displayed by antibodies against related flaviviruses, such as West Nile virus (WNV), Yellow Fever virus (YFV) or Tick-borne encephalitis virus (TBEV). Especially in areas where several flaviviruses co-circulate or in the context of vaccination e.g. against YFV or TBEV, this severely complicates diagnosis and surveillance. Most flavivirus cross-reactive antibodies are produced against the highly conserved fusion loop (FL) domain in the viral envelope (E) protein. We generated insect-cell derived recombinant E-proteins of the four DENV-serotypes which contain point mutations in the FL domain. By using specific mixtures of these mutant antigens, cross-reactivity against heterologous flaviviruses was strongly reduced, enabling sensitive and specific diagnosis of the DENV-infected serum samples in IgG and IgM-measurements. These results have indications for the development of serological DENV-tests with improved specificity.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectCell Lineeng
dc.subjectSensitivity and Specificityeng
dc.subjectCross Reactionseng
dc.subjectRecombinant Proteins/geneticseng
dc.subjectAntibodies Viral/bloodeng
dc.subjectDengue/diagnosiseng
dc.subjectDengue Virus/immunologyeng
dc.subjectImmunoglobulin G/bloodeng
dc.subjectImmunoglobulin M/bloodeng
dc.subjectDrosophilaeng
dc.subjectMutant Proteins/geneticseng
dc.subjectMutant Proteins/immunologyeng
dc.subjectRecombinant Proteins/immunologyeng
dc.subjectSerologic Tests/methodseng
dc.subjectViral Envelope Proteins/geneticseng
dc.subjectViral Envelope Proteins/immunologyeng
dc.subject.ddc610 Medizin
dc.titleRecombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10041592
dc.identifier.doi10.1371/journal.pntd.0004218
dc.identifier.doihttp://dx.doi.org/10.25646/2098
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.container-textRockstroh A, Barzon L, Pacenti M, Palù G, Niedrig M, Ulbert S (2015) Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections. PLoS Negl Trop Dis 9(11): e0004218.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004218
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume9
local.edoc.container-issue11
local.edoc.container-year2015

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