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2016-06-17Zeitschriftenartikel DOI: 10.1371/journal.pone.0157663
The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants
dc.contributor.authorKonrat, Katharina
dc.contributor.authorSchwebke, Ingeborg
dc.contributor.authorLaue, Michael
dc.contributor.authorDittmann, Christin
dc.contributor.authorLevin, Katja
dc.contributor.authorAndrich, Ricarda
dc.contributor.authorArvand, Mardjan
dc.contributor.authorSchaudinn, Christoph
dc.date.accessioned2018-05-07T19:08:30Z
dc.date.available2018-05-07T19:08:30Z
dc.date.created2016-07-26
dc.date.issued2016-06-17none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reAT848M6QY/PDF/29UihAhaXjAu.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2354
dc.description.abstractBacteria live primarily in microbial communities (biofilms), where they exhibit considerably higher biocide tolerance than their planktonic counterparts. Current standardized efficacy testing protocols of disinfectants, however, employ predominantly planktonic bacteria. In order to test the efficacy of biocides on biofilms in a standardized manner, a new assay was developed and optimized for easy-handling, quickness, low running costs, and above all—repeatability. In this assay, 5 mm glass- or polytetrafluoroethylene beads in 24 well microtiter plates served as substrate for Pseudomonas aeruginosa biofilms. After optimizing result-relevant steps, the actual performance of the assay was explored by treating P. aeruginosa biofilms with glutaraldehyde, isopropanol, or peracetic acid in predefined concentrations. The aspired 5 log10 reduction in CFU counts was achieved by glutaraldehyde at 5% (30 min), and by peracetic acid at 0.3% (10 min). In contrast, 80% isopropanol (30 min) failed to meet the reduction goal. However, the main accomplishment of this study was to unveil the potential of the array itself; most noteworthy here, a reliable repeatability of the results. The new bead assay for biofilms is a robust, quick and cost-effective method for assessing the efficacy of biocides against biofilms.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subject.ddc610 Medizin
dc.titleThe Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10045825
dc.identifier.doi10.1371/journal.pone.0157663
dc.identifier.doihttp://dx.doi.org/10.25646/2279
local.edoc.container-titlePLoS ONE
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157663
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume11
local.edoc.container-issue6
local.edoc.container-year2016

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