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2016-01-01Zeitschriftenartikel DOI: 10.1093/ve/vew016
Rapid outbreak sequencing of Ebola virus in Sierra Leone identifies transmission chains linked to sporadic cases
dc.contributor.authorArias, Armando
dc.contributor.authorWatson, Simon J.
dc.contributor.authorAsogun, Danny
dc.contributor.authorTobin, Ekaete Alice
dc.contributor.authorLu, Jia
dc.contributor.authorPhan, My V. T.
dc.contributor.authorJah, Umaru
dc.contributor.authorWadoum, Raoul Emeric Guetiya
dc.contributor.authorMeredith, Luke
dc.contributor.authorCaddy, Lucy Thorne Sarah
dc.contributor.authorTarawalie, Alimamy
dc.contributor.authorLangat, Pinky
dc.contributor.authorDudas, Gytis
dc.contributor.authorFaria, Nuno R.
dc.contributor.authorDellicour, Simon
dc.contributor.authorKamara, Abdul
dc.contributor.authorKargbo, Brima
dc.contributor.authorKamara, Brima Osaio
dc.contributor.authorGevao, Sahr
dc.contributor.authorCooper, Daniel
dc.contributor.authorNewport, Matthew
dc.contributor.authorKurth, Andreas
dc.contributor.authorKellam, Paul
dc.contributor.authorGoodfellow, Ian
dc.contributor.authorCotten, Matthew
dc.date.accessioned2018-05-07T20:23:39Z
dc.date.available2018-05-07T20:23:39Z
dc.date.created2017-08-25
dc.date.issued2016-01-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rey6SGaW3fbr2/PDF/24pGL9nBVSjYY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2760
dc.description.abstractTo end the largest known outbreak of Ebola virus disease (EVD) in West Africa and to prevent new transmissions, rapid epidemiological tracing of cases and contacts was required. The ability to quickly identify unknown sources and chains of transmission is key to ending the EVD epidemic and of even greater importance in the context of recent reports of Ebola virus (EBOV) persistence in survivors. Phylogenetic analysis of complete EBOV genomes can provide important information on the source of any new infection. A local deep sequencing facility was established at the Mateneh Ebola Treatment Centre in central Sierra Leone. The facility included all wetlab and computational resources to rapidly process EBOV diagnostic samples into full genome sequences. We produced 554 EBOV genomes from EVD cases across Sierra Leone. These genomes provided a detailed description of EBOV evolution and facilitated phylogenetic tracking of new EVD cases. Importantly, we show that linked genomic and epidemiological data can not only support contact tracing but also identify unconventional transmission chains involving body fluids, including semen. Rapid EBOV genome sequencing, when linked to epidemiological information and a comprehensive database of virus sequences across the outbreak, provided a powerful tool for public health epidemic control efforts.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjecttransmissioneng
dc.subjectevolutioneng
dc.subjectEbola viruseng
dc.subjectoutbreak sequencingeng
dc.subject.ddc610 Medizin
dc.titleRapid outbreak sequencing of Ebola virus in Sierra Leone identifies transmission chains linked to sporadic cases
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10054389
dc.identifier.doi10.1093/ve/vew016
dc.identifier.doihttp://dx.doi.org/10.25646/2685
local.edoc.container-titleVirus Evolution
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://academic.oup.com/ve/article/2/1/vew016/1753554/Rapid-outbreak-sequencing-of-Ebola-virus-in-Sierra
local.edoc.container-publisher-nameOxford University Press
local.edoc.container-volume2
local.edoc.container-issue1
local.edoc.container-year2016

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