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2017-11-10Zeitschriftenartikel DOI: 10.3390/v9110337
Combined Proteomics/Genomics Approach Reveals Proteomic Changes of Mature Virions as a Novel Poxvirus Adaptation Mechanism
dc.contributor.authorGrossegesse, Marica
dc.contributor.authorDöllinger, Jörg
dc.contributor.authorTyshaieva, Alona
dc.contributor.authorSchaade, Lars
dc.contributor.authorNitsche, Andreas
dc.date.accessioned2018-05-07T20:48:48Z
dc.date.available2018-05-07T20:48:48Z
dc.date.created2017-12-11
dc.date.issued2017-11-10none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rey5cuKEeqgc/PDF/23ypSFj0Otous.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2895
dc.description.abstractDNA viruses, like poxviruses, possess a highly stable genome, suggesting that adaptation of virus particles to specific cell types is not restricted to genomic changes. Cowpox viruses are zoonotic poxviruses with an extraordinarily broad host range, demonstrating their adaptive potential in vivo. To elucidate adaptation mechanisms of poxviruses, we isolated cowpox virus particles from a rat and passaged them five times in a human and a rat cell line. Subsequently, we analyzed the proteome and genome of the non-passaged virions and each passage. While the overall viral genome sequence was stable during passaging, proteomics revealed multiple changes in the virion composition. Interestingly, an increased viral fitness in human cells was observed in the presence of increased immunomodulatory protein amounts. As the only minor variant with increasing frequency during passaging was located in a viral RNA polymerase subunit and, moreover, most minor variants were found in transcription-associated genes, protein amounts were presumably regulated at transcription level. This study is the first comparative proteome analysis of virus particles before and after cell culture propagation, revealing proteomic changes as a novel poxvirus adaptation mechanism.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectcowpox viruseng
dc.subjectpoxviruseng
dc.subjectadaptationeng
dc.subjectproteomicseng
dc.subjectgenomicseng
dc.subjectcell cultureeng
dc.subjectpassagingeng
dc.subject.ddc610 Medizin
dc.titleCombined Proteomics/Genomics Approach Reveals Proteomic Changes of Mature Virions as a Novel Poxvirus Adaptation Mechanism
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10055949
dc.identifier.doi10.3390/v9110337
dc.identifier.doihttp://dx.doi.org/10.25646/2820
local.edoc.container-titleViruses
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.mdpi.com/1999-4915/9/11/337
local.edoc.container-publisher-nameMDPI
local.edoc.container-volume9
local.edoc.container-issue11
local.edoc.container-year2017

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