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2018-02-06Zeitschriftenartikel DOI: 10.3390/v10020066
Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation
dc.contributor.authorFiebig, Uwe
dc.contributor.authorAbicht, Jan-Michael
dc.contributor.authorMayr, Tanja
dc.contributor.authorLängin, Matthias
dc.contributor.authorBähr, Andrea
dc.contributor.authorGuethoff, Sonja
dc.contributor.authorFalkenau, Almuth
dc.contributor.authorWolf, Eckhard
dc.contributor.authorReichart, Bruno
dc.contributor.authorShibahara, Tomoyuki
dc.contributor.authorDenner, Joachim
dc.date.accessioned2018-05-07T21:04:17Z
dc.date.available2018-05-07T21:04:17Z
dc.date.created2018-02-09
dc.date.issued2018-02-06none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rent9Wbe082gk/PDF/24ekWA4lObY2.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2978
dc.description.abstractThe porcine cytomegalovirus (PCMV) is a herpesvirus that may pose a risk for xenotransplantation using pig cells, tissues, or organs. Here, three orthotopic pig heart transplantations into baboons were studied. To detect PCMV, a real-time PCR and a Western blot assay based on four PCMV protein sequences, including two tegument proteins, were used. The transmission of PCMV from the donor pig to the recipient baboon was found in two cases, despite PCMV not being detected in the blood of the donor pigs by real-time PCR. Although it was not in the blood, PCMV was detected in different organs of the donor pigs, and in sibling animals. Immunohistochemistry using an antiserum that is specific for PCMV detected virus protein-expressing cells in all of the organs of the recipient baboon, most likely representing disseminated pig cells. Therefore, for the first time, the distribution of PCMV in organs of the donor pigs and the recipient baboons was described. In addition, baboon cytomegalovirus (BaCMV) was found activated in the recipient, and a screening for hepatitis E virus (HEV) and porcine lymphotropic herpesviruses (PLHV) was performed. For the first time, a cross-reactivity between antibodies directed against PCMV and BaCMV was found.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectvirus transmissioneng
dc.subjectxenotransplantationeng
dc.subjectporcine cytomegaloviruseng
dc.subjectvirus safetyeng
dc.subject.ddc610 Medizin
dc.titleDistribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10057453
dc.identifier.doi10.3390/v10020066
dc.identifier.doihttp://dx.doi.org/10.25646/2903
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.mdpi.com/1999-4915/10/2/66
local.edoc.container-publisher-nameMDPI
local.edoc.container-volume10
local.edoc.container-issue2
local.edoc.container-year2018

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