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2018-01-30Zeitschriftenartikel DOI: 10.1371/journal.pone.0191873
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Acinetobacter baumannii among horses entering a veterinary teaching hospital: The contemporary "Trojan Horse"
dc.contributor.authorWalther, Birgit
dc.contributor.authorKlein, Katja-Sophia
dc.contributor.authorBarton, Ann-Kristin
dc.contributor.authorSemmler, Torsten
dc.contributor.authorHuber, Charlotte
dc.contributor.authorWolf, Silver Anthony
dc.contributor.authorTedin, Karsten
dc.contributor.authorMerle, Roswitha
dc.contributor.authorMitrach, Franziska
dc.contributor.authorGuenther, Sebastian
dc.contributor.authorLübke-Becker, Antina
dc.contributor.authorGehlen, Heidrun
dc.date.accessioned2018-05-07T21:04:29Z
dc.date.available2018-05-07T21:04:29Z
dc.date.created2018-02-12
dc.date.issued2018-01-30none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rent9Wbe082gk/PDF/20z1bMuKNFT9k.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2979
dc.description.abstractPathogens frequently associated with multi-drug resistant (MDR) phenotypes, including extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and Acinetobacter baumannii isolated from horses admitted to horse clinics, pose a risk for animal patients and personnel in horse clinics. To estimate current rates of colonization, a total of 341 equine patients were screened for carriage of zoonotic indicator pathogens at hospital admission. Horses showing clinical signs associated with colic (n = 233) or open wounds (n = 108) were selected for microbiological examination of nostril swabs, faecal samples and wound swabs taken from the open wound group. The results showed alarming carriage rates of Gram-negative MDR pathogens in equine patients: 10.7% (34 of 318) of validated faecal specimens were positive for ESBL-E (94%: ESBL-producing Escherichia coli), with recorded rates of 10.5% for the colic and 11% for the open wound group. 92.7% of the ESBL-producing E. coli were phenotypically resistant to three or more classes of antimicrobials. A. baumannii was rarely detected (0.9%), and all faecal samples investigated were negative for Salmonella, both directly and after two enrichment steps. Screening results for the equine nostril swabs showed detection rates for ESBL-E of 3.4% among colic patients and 0.9% in the open wound group, with an average rate of 2.6% (9/340) for both indications. For all 41 ESBL-producing E. coli isolated, a broad heterogeneity was revealed using pulsed-field gel electrophoresis (PFGE) patterns and whole genome sequencing (WGS) -analysis. However, a predominance of sequence type complex (STC)10 and STC1250 was observed, including several novel STs. The most common genes associated with ESBL-production were identified as blaCTX-M-1 (31/41; 75.6%) and blaSHV-12 (24.4%). The results of this study reveal a disturbingly large fraction of multi-drug resistant and ESBL-producing E. coli among equine patients, posing a clear threat to established hygiene management systems and work-place safety of veterinary staff in horse clinics.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subject.ddc610 Medizin
dc.titleExtended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Acinetobacter baumannii among horses entering a veterinary teaching hospital: The contemporary "Trojan Horse"
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10057468
dc.identifier.doi10.1371/journal.pone.0191873
dc.identifier.doihttp://dx.doi.org/10.25646/2904
local.edoc.container-titlePLoS ONE
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191873
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume13
local.edoc.container-issue1
local.edoc.container-year2018

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