Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Acinetobacter baumannii among horses entering a veterinary teaching hospital: The contemporary "Trojan Horse"
dc.contributor.author | Walther, Birgit | |
dc.contributor.author | Klein, Katja-Sophia | |
dc.contributor.author | Barton, Ann-Kristin | |
dc.contributor.author | Semmler, Torsten | |
dc.contributor.author | Huber, Charlotte | |
dc.contributor.author | Wolf, Silver Anthony | |
dc.contributor.author | Tedin, Karsten | |
dc.contributor.author | Merle, Roswitha | |
dc.contributor.author | Mitrach, Franziska | |
dc.contributor.author | Guenther, Sebastian | |
dc.contributor.author | Lübke-Becker, Antina | |
dc.contributor.author | Gehlen, Heidrun | |
dc.date.accessioned | 2018-05-07T21:04:29Z | |
dc.date.available | 2018-05-07T21:04:29Z | |
dc.date.created | 2018-02-12 | |
dc.date.issued | 2018-01-30 | none |
dc.identifier.other | http://edoc.rki.de/oa/articles/rent9Wbe082gk/PDF/20z1bMuKNFT9k.pdf | |
dc.identifier.uri | http://edoc.rki.de/176904/2979 | |
dc.description.abstract | Pathogens frequently associated with multi-drug resistant (MDR) phenotypes, including extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and Acinetobacter baumannii isolated from horses admitted to horse clinics, pose a risk for animal patients and personnel in horse clinics. To estimate current rates of colonization, a total of 341 equine patients were screened for carriage of zoonotic indicator pathogens at hospital admission. Horses showing clinical signs associated with colic (n = 233) or open wounds (n = 108) were selected for microbiological examination of nostril swabs, faecal samples and wound swabs taken from the open wound group. The results showed alarming carriage rates of Gram-negative MDR pathogens in equine patients: 10.7% (34 of 318) of validated faecal specimens were positive for ESBL-E (94%: ESBL-producing Escherichia coli), with recorded rates of 10.5% for the colic and 11% for the open wound group. 92.7% of the ESBL-producing E. coli were phenotypically resistant to three or more classes of antimicrobials. A. baumannii was rarely detected (0.9%), and all faecal samples investigated were negative for Salmonella, both directly and after two enrichment steps. Screening results for the equine nostril swabs showed detection rates for ESBL-E of 3.4% among colic patients and 0.9% in the open wound group, with an average rate of 2.6% (9/340) for both indications. For all 41 ESBL-producing E. coli isolated, a broad heterogeneity was revealed using pulsed-field gel electrophoresis (PFGE) patterns and whole genome sequencing (WGS) -analysis. However, a predominance of sequence type complex (STC)10 and STC1250 was observed, including several novel STs. The most common genes associated with ESBL-production were identified as blaCTX-M-1 (31/41; 75.6%) and blaSHV-12 (24.4%). The results of this study reveal a disturbingly large fraction of multi-drug resistant and ESBL-producing E. coli among equine patients, posing a clear threat to established hygiene management systems and work-place safety of veterinary staff in horse clinics. | eng |
dc.language.iso | eng | |
dc.publisher | Robert Koch-Institut | |
dc.subject.ddc | 610 Medizin | |
dc.title | Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Acinetobacter baumannii among horses entering a veterinary teaching hospital: The contemporary "Trojan Horse" | |
dc.type | periodicalPart | |
dc.identifier.urn | urn:nbn:de:0257-10057468 | |
dc.identifier.doi | 10.1371/journal.pone.0191873 | |
dc.identifier.doi | http://dx.doi.org/10.25646/2904 | |
local.edoc.container-title | PLoS ONE | |
local.edoc.fp-subtype | Artikel | |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
local.edoc.container-url | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191873 | |
local.edoc.container-publisher-name | Public Library of Science | |
local.edoc.container-volume | 13 | |
local.edoc.container-issue | 1 | |
local.edoc.container-year | 2018 |