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2008-09-11Zeitschriftenartikel DOI: 10.1186/1471-2180-8-144
Characterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep
dc.contributor.authorFröhlicher, Erik
dc.contributor.authorKrause, Gladys
dc.contributor.authorZweifel, Claudio
dc.contributor.authorBeutin, Lothar
dc.contributor.authorStephan, Roger
dc.date.accessioned2018-05-07T13:06:28Z
dc.date.available2018-05-07T13:06:28Z
dc.date.created2009-04-20
dc.date.issued2008-09-11none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re3dtx7lGJf32/PDF/25mEVoiuRBs.pdf
dc.identifier.urihttp://edoc.rki.de/176904/390
dc.description.abstractBackground: Attaching and effacing Escherichia coli (AEEC) are characterized by their ability to cause attaching-and-effacing (A/E) lesions in the gut mucosa of human and animal hosts leading to diarrhoea. The genetic determinants for the production of A/E lesions are located on the locus of enterocyte effacement (LEE), a pathogenicity island that also contains the genes encoding intimin (eae). This study reports data on the occurrence of eae positive E. coli carried by healthy pigs and sheep at the point of slaughter, and on serotypes, intimin variants, and further virulence factors of isolated AEEC strains. Results: Faecal samples from 198 finished pigs and 279 sheep were examined at slaughter. The proportion of eae positive samples was 89% for pigs and 55% for sheep. By colony dot-blot hybridization, AEEC were isolated from 50 and 53 randomly selected porcine and ovine samples and further characterized. Strains of the serotypes O2:H40, O3:H8 and O26:H11 were found in both pigs and sheep. In pigs O2:H40, O2:H49, O108:H9, O145:H28 and in sheep O2:H40, O26:H11, O70:H40, O146:H21 were the most prevalent serotypes among typable strains. Eleven different intimin types were detected, whereas γ2/θ was the most frequent, followed by β1, ε and γ1. All but two ovine strains tested negative for the genes encoding Shiga toxins. All strains tested negative for the bfpA gene and the EAF plasmid. EAST1 (astA) was present in 18 of the isolated strains. Conclusion: Our data show that pigs and sheep are a source of serologically and genetically diverse intimin-harbouring E. coli strains. Most of the strains show characteristics of atypical enteropathogenic E. coli. Nevertheless, there are stx-negative AEEC strains belonging to serotypes and intimin types that are associated with classical enterohaemorrhagic E. coli strains (O26:H11, β1; O145:H28, γ1).eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.rightsCreative Commons Namensnennung 3.0
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectAnimalseng
dc.subjectDNAeng
dc.subjectFeces/microbiologyeng
dc.subjectAdhesinseng
dc.subjectBacterial/geneticseng
dc.subjectDisease Reservoirs/veterinaryeng
dc.subjectEscherichia coli/classificationeng
dc.subjectEscherichia coli/geneticseng
dc.subjectEscherichia coli/isolation & purificationeng
dc.subjectEscherichia coli/pathogenicityeng
dc.subjectEscherichia coli Infections/microbiologyeng
dc.subjectEscherichia coli Infections/veterinaryeng
dc.subjectEscherichia coli Proteins/geneticseng
dc.subjectSerotypingeng
dc.subjectSheep/microbiologyeng
dc.subjectSheep Diseases/microbiologyeng
dc.subjectSwine/microbiologyeng
dc.subjectSwine Diseases/microbiologyeng
dc.subject.ddc610 Medizin
dc.titleCharacterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-100223
dc.identifier.doi10.1186/1471-2180-8-144
dc.identifier.doihttp://dx.doi.org/10.25646/315
local.edoc.container-titleBMC Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.biomedcentral.com/1471-2180/8/144
local.edoc.container-publisher-nameBioMed Central
local.edoc.container-volume8
local.edoc.container-issue144
local.edoc.container-year2008

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