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2005-01-24Zeitschriftenartikel DOI: 10.1186/1471-2180-5-4
Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48
dc.contributor.authorBeutin, Lothar
dc.contributor.authorStrauch, Eckhard
dc.contributor.authorZimmermann, Sonja
dc.contributor.authorKaulfuss, Stefan
dc.contributor.authorSchaudinn, Christoph
dc.contributor.authorMännel, Andrea
dc.contributor.authorGelderblom, Hans R.
dc.date.accessioned2018-05-07T13:16:56Z
dc.date.available2018-05-07T13:16:56Z
dc.date.created2009-09-03
dc.date.issued2005-01-24none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/revISvcJvpmSw/PDF/20zqywjcHYpbk.pdf
dc.identifier.urihttp://edoc.rki.de/176904/446
dc.description.abstractBackground: Serotyping of O-(lipopolysaccharide) and H-(flagellar) antigens is a wideley used method for identification of pathogenic strains and clones of Escherichia coli. At present, 176 O- and 53 H-antigens are described for E. coli which occur in different combinations in the strains. The flagellar antigen H4 is widely present in E. coli strains of different O-serotypes and pathotypes and we have investigated the genetic relationship between H4 encoding fliC genes by PCR, nucleotide sequencing and expression studies. Results: The complete nucleotide sequence of fliC genes present in E. coli reference strains U9-41 (O2:K1:H4) and P12b (O15:H17) was determined and both were found 99.3% (1043 of 1050 nucleotides) identical in their coding sequence. A PCR/RFLP protocol was developed for typing of fliC-H4 strains and 88 E. coli strains reacting with H4 antiserum were investigated. Nucleotide sequencing of complete fliC genes of six E. coli strains which were selected based on serum agglutination titers, fliC-PCR genotyping and reference data revealed 96.6 to 100% identity on the amino acid level. The functional expression of flagellin encoded by fliC-H4 from strain U9-41 and from our strain P12b which is an H4 expressing variant type was investigated in the E. coli K-12 strain JM109 which encodes flagellar type H48. The fliC recombinant plasmid carrying JM109 strains reacted with both H4 and H48 specific antisera whereas JM109 reacted only with the H48 antiserum. By immunoelectron microscopy, we could show that the flagella made by the fliC-H4 recombinant plasmid carrying strain are constituted of H48 and H4 flagellins which are coassembled into functional flagella. Conclusion: The flagellar serotype H4 is encoded by closely related fliC genes present in serologically different types of E. coli strainswhich were isolated at different time periods and geographical locations. Our expression studies show for the first time, that flagellins of different molecular weigth are functionally expressed and coassembled in the same flagellar filament in E. coli.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectBacterial/geneticseng
dc.subjectAmino Acid Sequenceeng
dc.subjectMoleculareng
dc.subjectBacterial/chemistryeng
dc.subjectBacterialeng
dc.subjectAntigenseng
dc.subjectCloningeng
dc.subjectEscherichia coli/classificationeng
dc.subjectEscherichia coli/cytologyeng
dc.subjectEscherichia coli/geneticseng
dc.subjectEscherichia coli/metabolismeng
dc.subjectEscherichia coli Proteins/chemistryeng
dc.subjectEscherichia coli Proteins/geneticseng
dc.subjectEscherichia coli Proteins/metabolismeng
dc.subjectFlagellin/chemistryeng
dc.subjectFlagellin/geneticseng
dc.subjectFlagellin/metabolismeng
dc.subjectGene Expression Regulationeng
dc.subjectGeneseng
dc.subjectMolecular Sequence Dataeng
dc.subjectPhylogenyeng
dc.subjectSequence Alignmenteng
dc.subjectSequence Homologyeng
dc.subjectAmino Acideng
dc.subjectSerotypingeng
dc.subject.ddc610 Medizin
dc.titleGenetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-1001411
dc.identifier.doi10.1186/1471-2180-5-4
dc.identifier.doihttp://dx.doi.org/10.25646/371
local.edoc.container-titleBMC Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.biomedcentral.com/1471-2180/5/4
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume5
local.edoc.container-issue4
local.edoc.container-year2005

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