Adding to Yersinia enterocolitica Gene Pool Diversity: Two Cryptic Plasmids from a Biotype 1A Isolate
dc.contributor.author | Lepka, Daniela | |
dc.contributor.author | Kerrinnes, Tobias | |
dc.contributor.author | Skiebe, Evelyn | |
dc.contributor.author | Hahn, Birgitt | |
dc.contributor.author | Fruth, Angelika | |
dc.contributor.author | Wilharm, Gottfried | |
dc.date.accessioned | 2018-05-07T13:21:57Z | |
dc.date.available | 2018-05-07T13:21:57Z | |
dc.date.created | 2009-11-03 | |
dc.date.issued | 2009-07-13 | none |
dc.identifier.other | http://edoc.rki.de/oa/articles/rectxlDXpq9jE/PDF/21OBABnd67Hg6.pdf | |
dc.identifier.uri | http://edoc.rki.de/176904/473 | |
dc.description.abstract | We report the nucleotide sequence of two novel cryptic plasmids (4357 and 14 662 base pairs) carried by a Yersinia enterocolitica biotype 1A strain isolated from pork. As distinguished from most biotype 1A strains, this isolate, designated 07-04449, exhibited adherence to eukaryotic cells. The smaller plasmid pYe4449-1 carries five attributable open reading frames (ORFs) encoding the first CcdA/CcdB-like antitoxin/toxin system described for a Yersinia plasmid, a RepA-like replication initiation protein, and mobilizing factors MobA and MobC. The deduced amino acid sequences showed highest similarity to proteins described in Salmonella (CcdA/B), Klebsiella (RepA), and Plesiomonas (MobA/C) indicating genomic fluidity among members of the Enterobacteriaceae. One additional ORF with unknown function, termed ORF5, was identified with an ancestry distinct from the rest of the plasmid. While the C+G content of ORF5 is 38.3%, the rest of pYe4449-1 shows a C+G content of 55.7%. The C+G content of the larger plasmid pYe4449-2 (54.9%) was similar to that of pYe4449-1 (53.7%) and differed from that of the Y. enterocolitica genome (47.3%). Of the 14 ORFs identified on pYe4449-2, only six ORFs showed significant similarity to database entries. For three of these ORFs likely functions could be ascribed: a TnpR-like resolvase and a phage replication protein, localized each on a low C+G island, and DNA primase TraC. Two ORFs of pYe4449-2, ORF3 and ORF7, seem to encode secretable proteins. Epitope-tagging of ORF3 revealed protein expression at 4°C but not at or above 27°C suggesting adaptation to a habitat outside swine. The hypothetical protein encoded by ORF7 is the member of a novel repeat protein family sharing the DxxGN(x)nDxxGN motif. Our findings illustrate the exceptional gene pool diversity within the species Y. enterocolitica driven by horizontal gene transfer events. | eng |
dc.language.iso | eng | |
dc.publisher | Robert Koch-Institut | |
dc.subject | Yersinia enterocolitica | eng |
dc.subject | biotype 1A | eng |
dc.subject | cryptic plasmid | eng |
dc.subject | virulence | eng |
dc.subject | yersinia secretable repeat protein | eng |
dc.subject | YsrA | eng |
dc.subject | horizontal gene transfer | eng |
dc.subject | Yersinia low temperature protein A | eng |
dc.subject | YltA | eng |
dc.subject.ddc | 610 Medizin | |
dc.title | Adding to Yersinia enterocolitica Gene Pool Diversity: Two Cryptic Plasmids from a Biotype 1A Isolate | |
dc.type | periodicalPart | |
dc.identifier.urn | urn:nbn:de:0257-1002730 | |
dc.identifier.doi | 10.1155/2009/398434 | |
dc.identifier.doi | http://dx.doi.org/10.25646/398 | |
local.edoc.container-title | Journal of Biomedicine and Biotechnology | |
local.edoc.fp-subtype | Artikel | |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
local.edoc.container-url | http://www.hindawi.com/journals/jbb/2009/398434.html | |
local.edoc.container-publisher-name | Hindawi Publishing Corporation | |
local.edoc.container-volume | 2009 | |
local.edoc.container-year | 2009 |