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2018-08-09Zeitschriftenartikel DOI: 10.25646/5663
Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany
dc.contributor.authorPietsch, Michael
dc.contributor.authorIrrgang, Alexandra
dc.contributor.authorRoschanski, Nicole
dc.contributor.authorBrenner Michael, Geovana
dc.contributor.authorHamprecht, Axel
dc.contributor.authorRieber, Heime
dc.contributor.authorKäsbohrer, Annemarie
dc.contributor.authorSchwarz, Stefan
dc.contributor.authorRösler, Uwe
dc.contributor.authorKreienbrock, Lothar
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorFuchs, Stephan
dc.contributor.authorWerner, Guido
dc.date.accessioned2018-09-11T07:17:10Z
dc.date.available2018-09-11T07:17:10Z
dc.date.issued2018-08-09none
dc.identifier.other10.1186/s12864-018-4976-3
dc.identifier.urihttp://edoc.rki.de/176904/5728
dc.description.abstractBackground: Resistance to 3rd-generation cephalosporins in Escherichia coli is mostly mediated by extended-spectrum beta-lactamases (ESBLs) or AmpC beta-lactamases. Besides overexpression of the species-specific chromosomal ampC gene, acquisition of plasmid-encoded ampC genes, e.g. blaCMY-2, has been described worldwide in E. coli from humans and animals. To investigate a possible transmission of blaCMY-2 along the food production chain, we conducted a next-generation sequencing (NGS)-based analysis of 164 CMY-2-producing E. coli isolates from humans, livestock animals and foodstuff from Germany. Results: The data of the 164 sequenced isolates revealed 59 different sequence types (STs); the most prevalent ones were ST38 (n = 19), ST131 (n = 16) and ST117 (n = 13). Two STs were present in all reservoirs: ST131 (human n = 8; food n = 2; animal n = 6) and ST38 (human n = 3; animal n = 9; food n = 7). All but one CMY-2-producing ST131 isolates belonged to the clade B (fimH22) that differed substantially from the worldwide dominant CTX-M-15-producing clonal lineage ST131-O25b clade C (fimH30). Plasmid replicon types IncI1 (n = 61) and IncK (n = 72) were identified for the majority of blaCMY-2-carrying plasmids. Plasmid sequence comparisons showed a remarkable sequence identity, especially for IncK plasmids. Associations of replicon types and distinct STs were shown for IncK and ST57, ST429 and ST38 as well as for IncI1 and ST58. Additional β-lactamase genes (blaTEM, blaCTX-M, blaOXA, blaSHV) were detected in 50% of the isolates, and twelve E. coli from chicken and retail chicken meat carried the colistin resistance gene mcr-1. Conclusion: We found isolates of distinct E. coli clonal lineages (ST131 and ST38) in all three reservoirs. However, a direct clonal relationship of isolates from food animals and humans was only noticeable for a few cases. The CMY-2-producing E. coli-ST131 represents a clonal lineage different from the CTX-M-15-producing ST131-O25b cluster. Apart from the ST-driven spread, plasmid-mediated spread, especially via IncI1 and IncK plasmids, likely plays an important role for emergence and transmission of blaCMY-2 between animals and humans.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectMultidrug-resistanteng
dc.subjectFood chaineng
dc.subjectPlasmideng
dc.subjectAmpC beta-lactamaseeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleWhole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germanynone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5728-0
dc.identifier.doihttp://dx.doi.org/10.25646/5663
dc.type.versionpublishedVersionnone
local.edoc.container-titleBMC Genomicsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4976-3none
local.edoc.container-publisher-nameBioMedCentralnone
local.edoc.container-volume19none
local.edoc.container-issue601none
local.edoc.container-reportyear2018none
local.edoc.container-firstpage1none
local.edoc.container-lastpage17none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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