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2018-10-03Zeitschriftenartikel DOI: 10.25646/5921
ESBL-Producing Klebsiella pneumoniae in the Broiler Production Chain and the First Description of ST3128
dc.contributor.authorDaehre, Katrin
dc.contributor.authorProjahn, Michaela
dc.contributor.authorFriese, Anika
dc.contributor.authorSemmler, Torsten
dc.contributor.authorGuenther, Sebastian
dc.contributor.authorRoesler, Uwe H.
dc.date.accessioned2019-03-05T07:23:04Z
dc.date.available2019-03-05T07:23:04Z
dc.date.issued2018-10-03none
dc.identifier.other10.3389/fmicb.2018.02302
dc.identifier.urihttp://edoc.rki.de/176904/5956
dc.description.abstractESBL-producing Klebsiella pneumoniae (K. pneumoniae) represent an increasing problem both in human and veterinary medicine. As SHV-2 - encoding K. pneumoniae were recently detected in the broiler production we were interested in investigating a possible transmission along the broiler production chain and furthermore, in evaluating their possible impact on human health. Therefore, 41 ESBL-producing K. pneumoniae originating from a parent flock, from the hatcherys' environment during the hatching of that parent flocks' chickens, and from an associated fattening flock were investigated on an Illumina Miseq. Whole genome sequences were analyzed concerning their MLST-type, cgMLST-type, genotypic and phenotypic resistance, plasmid profiles and virulence genes. Irrespective of the origin of isolation all investigated isolates were multi-drug resistant, harbored the same ESBL-gene blaSHV−2, shared the same sequence type (ST3128) and displayed 100% similarity in core genome multilocus sequence typing (cgMLST). In addition, in silico plasmid typing found several Inc/Rep types associated with ESBL-plasmids. Summarizing, identical clones of SHV-2—producing K. pneumoniae were detected in different stages of the industrial broiler production in one out of seven investigated broiler chains. This proves the possibility of pseudo-vertical transmission of multi-resistant human pathogens from parent flocks to hatcheries and fattening flocks. Furthermore, the importance of cross-contamination along the production chain was shown. Although the ESBL-producing K. pneumoniae clone detected here in the broiler production has not been associated with clinical settings so far, our findings present a potential public health threat.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectextended-spectrum-beta-lactamaseseng
dc.subjectESBLseng
dc.subjectKlebsiella pneumoniaeeng
dc.subjectbroiler productioneng
dc.subjectbroiler chickeneng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleESBL-Producing Klebsiella pneumoniae in the Broiler Production Chain and the First Description of ST3128none
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5956-2
dc.identifier.doihttp://dx.doi.org/10.25646/5921
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Microbiologynone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02302/fullnone
local.edoc.container-publisher-nameFrontiers Medianone
local.edoc.container-volume2018none
local.edoc.container-issue9none
local.edoc.container-reportyear2018none
local.edoc.container-year2018none
local.edoc.container-firstpage1none
local.edoc.container-lastpage6none
local.edoc.rki-departmentProjektgruppen/Nachwuchsgruppennone
dc.description.versionPeer Reviewednone

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