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2019-02-07Zeitschriftenartikel DOI: 10.25646/5947
Analysis of long non-coding RNA and mRNA expression in bovine macrophages brings up novel aspects of Mycobacterium avium subspecies paratuberculosis infections
dc.contributor.authorGupta, Pooja
dc.contributor.authorPeter, Sarah
dc.contributor.authorJung, Markus
dc.contributor.authorLewin, Astrid
dc.contributor.authorHemmrich-Stanisak, Georg
dc.contributor.authorFranke, Andre
dc.contributor.authorvon Kleist, Max
dc.contributor.authorSchütte, Christof
dc.contributor.authorEinspanier, Ralf
dc.contributor.authorSharbati, Soroush
dc.contributor.authorzur Bruegge, Jennifer
dc.date.accessioned2019-03-14T09:01:29Z
dc.date.available2019-03-14T09:01:29Z
dc.date.issued2019-02-07none
dc.identifier.other10.1038/s41598-018-38141-x
dc.identifier.urihttp://edoc.rki.de/176904/5982
dc.description.abstractParatuberculosis is a major disease in cattle that severely affects animal welfare and causes huge economic losses worldwide. Development of alternative diagnostic methods is of urgent need to control the disease. Recent studies suggest that long non-coding RNAs (lncRNAs) play a crucial role in regulating immune function and may confer valuable information about the disease. However, their role has not yet been investigated in cattle with respect to infection towards Paratuberculosis. Therefore, we investigated the alteration in genomic expression profiles of mRNA and lncRNA in bovine macrophages in response to Paratuberculosis infection using RNA-Seq. We identified 397 potentially novel lncRNA candidates in macrophages of which 38 were differentially regulated by the infection. A total of 820 coding genes were also significantly altered by the infection. Co-expression analysis of lncRNAs and their neighbouring coding genes suggest regulatory functions of lncRNAs in pathways related to immune response. For example, this included protein coding genes such as TNIP3, TNFAIP3 and NF-κB2 that play a role in NF-κB2 signalling, a pathway associated with immune response. This study advances our understanding of lncRNA roles during Paratuberculosis infection.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectBacterial infectioneng
dc.subjectComputational biology and bioinformaticseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleAnalysis of long non-coding RNA and mRNA expression in bovine macrophages brings up novel aspects of Mycobacterium avium subspecies paratuberculosis infectionsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5982-6
dc.identifier.doihttp://dx.doi.org/10.25646/5947
dc.type.versionpublishedVersionnone
local.edoc.container-titleScientific Reportsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41598-018-38141-x#Sec1none
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume9none
local.edoc.container-issue1571none
local.edoc.container-reportyear2019none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage14none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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