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2018-02-06Zeitschriftenartikel DOI: 10.25646/6051
Recommendations for enterovirus diagnostics and characterisation within and beyond Europe
dc.contributor.authorHarvala, Heli
dc.contributor.authorBroberg, Eeva
dc.contributor.authorBenschop, Kimberley
dc.contributor.authorBerginc, Natasa
dc.contributor.authorLadhani, Shamez
dc.contributor.authorSusi, Petri
dc.contributor.authorChristiansen, Claus
dc.contributor.authorMcKenna, James
dc.contributor.authorAllen, David
dc.contributor.authorMakiello, Phoebe
dc.contributor.authorMcAllister, Georgina
dc.contributor.authorCarmen, Mirabelli
dc.contributor.authorZakikhany, Katherina
dc.contributor.authorDyrdak, Robert
dc.contributor.authorNielsen, Xiaohui
dc.contributor.authorMadsen, Tina
dc.contributor.authorPaul, Joel
dc.contributor.authorMoore, Catherine
dc.contributor.authorvon Eije, Karin
dc.contributor.authorPiralla, Antonio
dc.contributor.authorCarlier, Mieke
dc.contributor.authorVanoverschelde, Laura
dc.contributor.authorPoelman, Randy
dc.contributor.authorAnton, Andrés
dc.contributor.authorLópez-Labrador, F. Xavier
dc.contributor.authorPellegrinelli, Laura
dc.contributor.authorKeeren, Kathrin
dc.contributor.authorMaier, Melanie
dc.contributor.authorCassidy, Hayley
dc.contributor.authorDerdas, Stavros
dc.contributor.authorSavolainen-Kopra, Carita
dc.contributor.authorDiedrich, Sabine
dc.contributor.authorNordbø, Svein
dc.contributor.authorBuesa, Javier
dc.contributor.authorBailly, Jean-Luc
dc.contributor.authorBaldanti, Fausto
dc.contributor.authorMacAdam, Andrew
dc.contributor.authorMirand, Audrey
dc.contributor.authorDudman, Susanne
dc.contributor.authorSchuffenecker, Isabelle
dc.contributor.authorKadambari, Seilesh
dc.contributor.authorNeyts, Johan
dc.contributor.authorGriffiths, Michael J.
dc.contributor.authorRichter, Jan
dc.contributor.authorMargaretto, Christina
dc.contributor.authorGovind, Sheila
dc.contributor.authorMorley, Ursula
dc.contributor.authorAdams, Ortwin
dc.contributor.authorKrokstad, Sidsel
dc.contributor.authorDean, Jonathan
dc.contributor.authorPons-Salort, Margarita
dc.contributor.authorProchazka, Birgit
dc.contributor.authorCabrerizo, Maria
dc.contributor.authorMajumdar, Manasi
dc.contributor.authorNebbia, Gaia
dc.contributor.authorWiewel, Maryse
dc.contributor.authorCottrell, Simon
dc.contributor.authorCoyle, Peter
dc.contributor.authorMartin, Javier
dc.contributor.authorMoore, Catrin
dc.contributor.authorMidgley, Sofie
dc.contributor.authorHorby, Peter
dc.contributor.authorWolthers, Katja
dc.contributor.authorSimmonds, Peter
dc.contributor.authorNiesters, Hubert
dc.contributor.authorFischer, Thea K.
dc.date.accessioned2019-04-09T07:54:29Z
dc.date.available2019-04-09T07:54:29Z
dc.date.issued2018-02-06none
dc.identifier.other10.1016/j.jcv.2018.01.008
dc.identifier.urihttp://edoc.rki.de/176904/6086
dc.description.abstractEnteroviruses (EV) can cause severe neurological and respiratory infections, and occasionally lead to devastating outbreaks as previously demonstrated with EV-A71 and EV-D68 in Europe. However, these infections are still often underdiagnosed and EV typing data is not currently collected at European level. In order to improve EV diagnostics, collate data on severe EV infections and monitor the circulation of EV types, we have established European non-polio enterovirus network (ENPEN). First task of this cross-border network has been to ensure prompt and adequate diagnosis of these infections in Europe, and hence we present recommendations for non-polio EV detection and typing based on the consensus view of this multidisciplinary team including experts from over 20 European countries. We recommend that respiratory and stool samples in addition to cerebrospinal fluid (CSF) and blood samples are submitted for EV testing from patients with suspected neurological infections. This is vital since viruses like EV-D68 are rarely detectable in CSF or stool samples. Furthermore, reverse transcriptase PCR (RT-PCR) targeting the 5′noncoding regions (5′NCR) should be used for diagnosis of EVs due to their sensitivity, specificity and short turnaround time. Sequencing of the VP1 capsid protein gene is recommended for EV typing; EV typing cannot be based on the 5′NCR sequences due to frequent recombination events and should not rely on virus isolation. Effective and standardized laboratory diagnostics and characterisation of circulating virus strains are the first step towards effective and continuous surveillance activities, which in turn will be used to provide better estimation on EV disease burden.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectEnteroviruseng
dc.subjectDetectioneng
dc.subjectEV typingeng
dc.subjectNeurological infectioneng
dc.subjectEuropean non-polio enterovirus network (ENPEN)eng
dc.subjectDiagnosticseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleRecommendations for enterovirus diagnostics and characterisation within and beyond Europenone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6086-9
dc.identifier.doihttp://dx.doi.org/10.25646/6051
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Clinical Virologynone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.sciencedirect.com/science/article/pii/S1386653218300088none
local.edoc.container-publisher-nameElseviernone
local.edoc.container-volume101none
local.edoc.container-issueApril 2018none
local.edoc.container-reportyear2018none
local.edoc.container-year2018none
local.edoc.container-firstpage11none
local.edoc.container-lastpage17none
local.edoc.rki-departmentInfektionsepidemiologienone
dc.description.versionPeer Reviewednone

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