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2016-12-10Zeitschriftenartikel DOI: 10.25646/6052
Persistence and clearance of Ebola virus RNA from seminal fluid of Ebola virus disease survivors: a longitudinal analysis and modelling study
dc.contributor.authorSissoko, Daouda
dc.contributor.authorDuraffour, Sophie
dc.contributor.authorKerber, Romy
dc.contributor.authorKolie, Jacques Seraphin
dc.contributor.authorHabib Beavogui, Abdoul
dc.contributor.authorCamara, Alseny Modet
dc.contributor.authorColin Pharm, Géraldine
dc.contributor.authorRieger, Toni
dc.contributor.authorOestereich, Lisa
dc.contributor.authorPályi, Bernadett
dc.contributor.authorWurr, Stephanie
dc.contributor.authorGuedj, Jeremie
dc.contributor.authorTram Nguyen, Thi Huyen
dc.contributor.authorEggo, Rosalind M.
dc.contributor.authorWatson, Conall H.
dc.contributor.authorEdmunds, John
dc.contributor.authorAkoi Bore, Joseph
dc.contributor.authorRaymond Koundouno, Fara
dc.contributor.authorCabeza-Cabrerizo, Mar
dc.contributor.authorCarter, Lisa L.
dc.contributor.authorKafetzopoulou, Liana Eleni
dc.contributor.authorKuisma, Eeva
dc.contributor.authorMichel, Janine
dc.contributor.authorPatrono, Livia V.
dc.contributor.authorRickett, Natasha Y.
dc.contributor.authorSingethan, Katrin
dc.contributor.authorRudolf, Martin
dc.contributor.authorLander, Angelika
dc.contributor.authorPallasch, Elisa
dc.contributor.authorBockholt, Sabrina
dc.contributor.authorRodríguez, Estefanía
dc.contributor.authorDi Caro, Antonino
dc.contributor.authorWölfel, Roman
dc.contributor.authorGabriel, Martin
dc.contributor.authorGurry, Céline
dc.contributor.authorFormenty, Pierre
dc.contributor.authorKeïta, Sakoba
dc.contributor.authorMalvy, Denis
dc.contributor.authorCarroll, Miles
dc.contributor.authorAnglaret, Xavier
dc.contributor.authorGünther, Stephan
dc.date.accessioned2019-04-09T08:49:40Z
dc.date.available2019-04-09T08:49:40Z
dc.date.issued2016-12-10none
dc.identifier.other10.1016/S2214-109X(16)30243-1
dc.identifier.urihttp://edoc.rki.de/176904/6087
dc.description.abstractBackground By January, 2016, all known transmission chains of the Ebola virus disease (EVD) outbreak in west Africa had been stopped. However, there is concern about persistence of Ebola virus in the reproductive tract of men who have survived EVD. We aimed to use biostatistical modelling to describe the dynamics of Ebola virus RNA load in seminal fluid, including clearance parameters. Methods In this longitudinal study, we recruited men who had been discharged from three Ebola treatment units in Guinea between January and July, 2015. Participants provided samples of seminal fluid at follow-up every 3–6 weeks, which we tested for Ebola virus RNA using quantitative real-time RT-PCR. Representative specimens from eight participants were then inoculated into immunodeficient mice to test for infectivity. We used a linear mixed-effect model to analyse the dynamics of virus persistence in seminal fluid over time. Findings We enrolled 26 participants and tested 130 seminal fluid specimens; median follow up was 197 days (IQR 187–209 days) after enrolment, which corresponded to 255 days (228–287) after disease onset. Ebola virus RNA was detected in 86 semen specimens from 19 (73%) participants. Median duration of Ebola virus RNA detection was 158 days after onset (73–181; maximum 407 days at end of follow-up). Mathematical modelling of the quantitative time-series data showed a mean clearance rate of Ebola virus RNA from seminal fluid of −0·58 log units per month, although the clearance kinetic varied greatly between participants. Using our biostatistical model, we predict that 50% and 90% of male survivors clear Ebola virus RNA from seminal fluid at 115 days (90% prediction interval 72–160) and 294 days (212–399) after disease onset, respectively. We also predicted that the number of men positive for Ebola virus RNA in affected countries would decrease from about 50 in January 2016, to fewer than 1 person by July, 2016. Infectious virus was detected in 15 of 26 (58%) specimens tested in mice. Interpretation Time to clearance of Ebola virus RNA from seminal fluid varies greatly between individuals and could be more than 13 months. Our predictions will assist in decision-making about surveillance and preventive measures in EVD outbreaks. Funding This study was funded by European Union's Horizon 2020 research and innovation programme, Directorate-General for International Cooperation and Development of the European Commission, Institut national de la santé et de la recherche médicale (INSERM), German Research Foundation (DFG), and Innovative Medicines Initiative 2 Joint Undertaking.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titlePersistence and clearance of Ebola virus RNA from seminal fluid of Ebola virus disease survivors: a longitudinal analysis and modelling studynone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6087-5
dc.identifier.doihttp://dx.doi.org/10.25646/6052
dc.type.versionpublishedVersionnone
local.edoc.container-titleThe Lancet Global Healthnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.sciencedirect.com/science/article/pii/S2214109X16302431none
local.edoc.container-publisher-nameElseviernone
local.edoc.container-volume5none
local.edoc.container-issue1none
local.edoc.container-reportyear2016none
local.edoc.container-year2016none
local.edoc.container-firstpagee80none
local.edoc.container-lastpagee88none
local.edoc.rki-departmentZentrum für Biologische Gefahren und Spezielle Pathogenenone
dc.description.versionPeer Reviewednone

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