Zur Kurzanzeige

2019-12-03Zeitschriftenartikel DOI: 10.25646/6559
Genomic and Functional Characterization of Poultry Escherichia coli From India Revealed Diverse Extended-Spectrum β-Lactamase-Producing Lineages With Shared Virulence Profiles
dc.contributor.authorHussain, Arif
dc.contributor.authorShaik, Sabiha
dc.contributor.authorRanjan, Amit
dc.contributor.authorSuresh, Arya
dc.contributor.authorSarker, Nishat
dc.contributor.authorSemmler, Torsten
dc.contributor.authorWieler, Lothar H.
dc.contributor.authorAlam, Munirul
dc.contributor.authorWatanabe, Haruo
dc.contributor.authorChakravortty, Dipshikha
dc.contributor.authorAhmed, Niyaz
dc.date.accessioned2020-03-18T10:19:58Z
dc.date.available2020-03-18T10:19:58Z
dc.date.issued2019-12-03none
dc.identifier.other10.3389/fmicb.2019.02766
dc.identifier.urihttp://edoc.rki.de/176904/6527
dc.description.abstractExtended-spectrum β-lactamases (ESBLs) form the most important resistance determinants prevalent worldwide. Data on ESBL-producing Escherichia coli from poultry and livestock are scarce in India. We present data on the functional and genomic characterization of ESBL-producing E. coli obtained from poultry in India. The whole genome sequences of 28 ESBL-producing E. coli were analyzed comprising of 12 broiler chicken E. coli isolates, 11 free-range chicken E. coli isolates, and 5 human extraintestinal pathogenic E. coli. All of the 28 ESBL-producing E. coli isolates were tested for antibiotic susceptibilities, in vitro conjugation, and virulence-associated phenotypic characteristics. A total of 13 sequence types were identified from the poultry E. coli, which included globally successful sequence types such as ST117 (9%), ST131 (4.3%), and ST10 (4.3%). The most common ESBL gene detected in poultry E. coli genomes was blaCTX-M-15 (17%). Also, FIB (73%) and FII (73%) were the most common plasmid replicons identified. Conjugation experiments demonstrated 54 (7/13), 30 (3/10), and 40% (2/5) of broiler, free-range, and human ExPEC E. coli to be able to transfer their ESBL genes, respectively. The in vitro virulence-associated phenotypic tests revealed the broiler, free-range, and human ExPEC isolates to be comparable in biofilm formation, resistance to serum bactericidal activity, adherence, and invasion capabilities. Our overall results showed prevalence of virulence phenotypes among the diverse ESBL-producing E. coli from poultry; while certain E. coli clones from broiler-poultry may indeed have the potential to cause infection in humans.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectpoultry Escherichia colieng
dc.subjectgenomicseng
dc.subjectESBLeng
dc.subjectExPECeng
dc.subjectIndiaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleGenomic and Functional Characterization of Poultry Escherichia coli From India Revealed Diverse Extended-Spectrum β-Lactamase-Producing Lineages With Shared Virulence Profilesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6527-9
dc.identifier.doihttp://dx.doi.org/10.25646/6559
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Microbiologynone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02766/fullnone
local.edoc.container-publisher-nameFrontiers Medianone
local.edoc.container-volume10none
local.edoc.container-issue2766none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage12none
local.edoc.rki-departmentProjektgruppen/Nachwuchsgruppennone
dc.description.versionPeer Reviewednone

Zur Kurzanzeige