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2020-02-28Zeitschriftenartikel DOI: 10.25646/6581
Whole genome sequencing reveals extended natural transformation in Campylobacter impacting diagnostics and the pathogens adaptive potential
dc.contributor.authorGolz, Julia C.
dc.contributor.authorEpping, Lennard
dc.contributor.authorKnüver, Marie-Theres
dc.contributor.authorBorowiak, Maria
dc.contributor.authorHartkopf, Felix
dc.contributor.authorDeneke, Carlus
dc.contributor.authorMalorny, Burkhard
dc.contributor.authorSemmler, Torsten
dc.contributor.authorStingl, Kerstin
dc.date.accessioned2020-03-23T08:33:47Z
dc.date.available2020-03-23T08:33:47Z
dc.date.issued2020-02-28none
dc.identifier.other10.1038/s41598-020-60320-y
dc.identifier.urihttp://edoc.rki.de/176904/6549
dc.description.abstractCampylobacter is the major bacterial agent of human gastroenteritis worldwide and represents a crucial global public health burden. Species differentiation of C. jejuni and C. coli and phylogenetic analysis is challenged by inter-species horizontal gene transfer. Routine real-time PCR on more than 4000 C. jejuni and C. coli field strains identified isolates with ambiguous PCR results for species differentiation, in particular, from the isolation source eggs. K-mer analysis of whole genome sequencing data indicated the presence of C. coli hybrid strains with huge amounts of C. jejuni introgression. Recombination events were distributed over the whole chromosome. MLST typing was impaired, since C. jejuni sequences were also found in six of the seven housekeeping genes. cgMLST suggested that the strains were phylogenetically unrelated. Intriguingly, the strains shared a stress response set of C. jejuni variant genes, with proposed roles in oxidative, osmotic and general stress defence, chromosome maintenance and repair, membrane transport, cell wall and capsular biosynthesis and chemotaxis. The results have practical impact on routine typing and on the understanding of the functional adaption to harsh environments, enabling successful spreading and persistence of Campylobacter.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectBacterial genomicseng
dc.subjectFood microbiologyeng
dc.subjectPathogenseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleWhole genome sequencing reveals extended natural transformation in Campylobacter impacting diagnostics and the pathogens adaptive potentialnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6549-4
dc.identifier.doihttp://dx.doi.org/10.25646/6581
dc.type.versionpublishedVersionnone
local.edoc.container-titleScientific Reportsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41598-020-60320-y#article-infonone
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume10none
local.edoc.container-issue3686none
local.edoc.container-year2020none
local.edoc.container-firstpage1none
local.edoc.container-lastpage12none
local.edoc.rki-departmentProjektgruppen/Nachwuchsgruppennone
dc.description.versionPeer Reviewednone

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