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2019-11-12Zeitschriftenartikel DOI: 10.25646/6624
Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR
dc.contributor.authorSpringer, Jan
dc.contributor.authorWalther, Grit
dc.contributor.authorRickerts, Volker
dc.contributor.authorHamprecht, Axel
dc.contributor.authorWillinger, Birgit
dc.contributor.authorTeschner, Daniel
dc.contributor.authorEinsele, Hermann
dc.contributor.authorKurzai, Oliver
dc.contributor.authorLoeffler, Juergen
dc.date.accessioned2020-03-31T10:04:46Z
dc.date.available2020-03-31T10:04:46Z
dc.date.issued2019-11-12none
dc.identifier.other10.3390/jof5040105
dc.identifier.urihttp://edoc.rki.de/176904/6584
dc.description.abstractThe mold Fusarium is a ubiquitous fungus causing plant, animal and human infections. In humans, Fusarium spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by Fusarium spp. mainly occur in immunosuppressed patients and can disseminate throughout the human body. Due to high levels of resistance to antifungals a fast identification of the causative agent is an urgent need. By using a probe-based real-time PCR assay specific for the genus Fusarium we analysed several different clinical specimens detecting Fusarium spp. commonly found in clinical samples in Germany. Also, a large collection of lung fluid samples of haematological patients was analysed (n = 243). In these, two samples (0.8%) were reproducibly positive, but only one could be confirmed by sequencing. For this case of probable invasive fungal disease (IFD) culture was positive for Fusarium species. Here we describe a rapid, probe-based real-time PCR assay to specifically detect DNA from a broad range of Fusarium species and its application to clinically relevant specimens.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectprobe-based real-time PCReng
dc.subjectFusariumeng
dc.subjectbronchoalveolar lavage fluideng
dc.subjectfungal molecular diagnosticseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDetection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCRnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6584-5
dc.identifier.doihttp://dx.doi.org/10.25646/6624
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Funginone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/2309-608X/5/4/105none
local.edoc.container-publisher-nameMolecular Diversity Preservation International (MDPI)none
local.edoc.container-volume5none
local.edoc.container-issue48none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage7none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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