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2020-03-12Zeitschriftenartikel DOI: 10.25646/6715
Adeno‐associated virus‐vectored influenza vaccine elicits neutralizing and Fcγ receptor‐activating antibodies
dc.contributor.authorDemminger, Daniel E.
dc.contributor.authorWalz, Lisa
dc.contributor.authorDietert, Kristina
dc.contributor.authorHoffmann, Helen
dc.contributor.authorPlanz, Oliver
dc.contributor.authorGruber, Achim D.
dc.contributor.authorvon Messling, Veronika
dc.contributor.authorWolff, Thorsten
dc.date.accessioned2020-04-14T08:37:30Z
dc.date.available2020-04-14T08:37:30Z
dc.date.issued2020-03-12none
dc.identifier.other10.15252/emmm.201910938
dc.identifier.urihttp://edoc.rki.de/176904/6663
dc.description.abstractThe current seasonal inactivated influenza vaccine protects only against a narrow range of virus strains as it triggers a dominant antibody response toward the hypervariable hemagglutinin (HA) head region. The discovery of rare broadly protective antibodies against conserved regions in influenza virus proteins has propelled research on distinct antigens and delivery methods to efficiently induce broad immunity toward drifted or shifted virus strains. Here, we report that adeno‐associated virus (AAV) vectors expressing influenza virus HA or chimeric HA protected mice against homologous and heterologous virus challenges. Unexpectedly, immunization even with wild‐type HA induced antibodies recognizing the HA‐stalk and activating FcγR‐dependent responses indicating that AAV‐vectored expression balances HA head‐ and HA stalk‐specific humoral responses. Immunization with AAV‐HA partially protected also ferrets against a harsh virus challenge. Results from this study provide a rationale for further clinical development of AAV vectors as influenza vaccine platform, which could benefit from their approved use in human gene therapy.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectadeno-associated virus vectoreng
dc.subjectbroadly reactive antibodyeng
dc.subjectHA stalkeng
dc.subjectuniversal influenza vaccineeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleAdeno‐associated virus‐vectored influenza vaccine elicits neutralizing and Fcγ receptor‐activating antibodiesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6663-4
dc.identifier.doihttp://dx.doi.org/10.25646/6715
dc.type.versionpublishedVersionnone
local.edoc.container-titleEMBO Molecular Medicinenone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.embopress.org/doi/full/10.15252/emmm.201910938none
local.edoc.container-publisher-nameEMBOpressnone
local.edoc.container-volume2020none
local.edoc.container-issuee10938none
local.edoc.container-year2020none
local.edoc.container-firstpage1none
local.edoc.container-lastpage18none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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