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2020-04-21Zeitschriftenartikel DOI: 10.25646/6989
Critical Role of Zur and SmtB in Zinc Homeostasis of Mycobacterium smegmatis
dc.contributor.authorGoethe, Elke
dc.contributor.authorLaarmann, Kristin
dc.contributor.authorLührs, Janita
dc.contributor.authorJarek, Michael
dc.contributor.authorMeens, Jochen
dc.contributor.authorLewin, Astrid
dc.contributor.authorGoethe, Ralph
dc.date.accessioned2020-07-03T12:13:55Z
dc.date.available2020-07-03T12:13:55Z
dc.date.issued2020-04-21none
dc.identifier.other10.1128/mSystems.00880-19
dc.identifier.urihttp://edoc.rki.de/176904/6870
dc.description.abstractZinc homeostasis is crucial for bacterial cells, since imbalances affect viability. However, in mycobacteria, knowledge of zinc metabolism is incomplete. Mycobacterium smegmatis (MSMEG) is an environmental, nonpathogenic Mycobacterium that is widely used as a model organism to study mycobacterial metabolism and pathogenicity. How MSMEG maintains zinc homeostasis is largely unknown. SmtB and Zur are important regulators of bacterial zinc metabolism. In mycobacteria, these regulators are encoded by an operon, whereas in other bacterial species, SmtB and Zur are encoded on separate loci. Here, we show that the smtB-zur operon is consistently present within the genus Mycobacterium but otherwise found only in Nocardia, Saccharothrix, and Corynebacterium diphtheriae. By RNA deep sequencing, we determined the Zur and SmtB regulons of MSMEG and compared them with transcriptional responses after zinc starvation or excess. We found an exceptional genomic clustering of genes whose expression was strongly induced by zur deletion and zinc starvation. These genes encoded zinc importers such as ZnuABC and three additional putative zinc transporters, including the porin MspD, as well as alternative ribosomal proteins. In contrast, only a few genes were affected by deletion of smtB and zinc excess. The zinc exporter ZitA was most prominently regulated by SmtB. Moreover, transcriptional analyses in combination with promoter and chromatin immunoprecipitation assays revealed a special regulation of the smtB-zur operon itself: an apparently zinc-independent, constitutive expression of smtB-zur resulted from sensitive coregulation by both SmtB and Zur. Overall, our data revealed yet unknown peculiarities of mycobacterial zinc homeostasis.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectzinc homeostasiseng
dc.subjectzinc transportereng
dc.subjectzinc starvationeng
dc.subjectzinc excesseng
dc.subjectZur reguloneng
dc.subjectSmtB reguloneng
dc.subjectzinc regulationeng
dc.subjectzitAeng
dc.subjectzinc importeng
dc.subjectzinc exporteng
dc.subjecttranscriptomicseng
dc.subjectmycobacteriaeng
dc.subjectznuABCeng
dc.subjectalternative ribosomal proteinseng
dc.subjectchromatin immunoprecipitationeng
dc.subjectcoregulationeng
dc.subjectexporteng
dc.subjectimporteng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleCritical Role of Zur and SmtB in Zinc Homeostasis of Mycobacterium smegmatisnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6870-3
dc.identifier.doihttp://dx.doi.org/10.25646/6989
dc.type.versionpublishedVersionnone
local.edoc.container-titlemSystemsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://msystems.asm.org/content/5/2/e00880-19none
local.edoc.container-publisher-nameASM Journalsnone
local.edoc.container-volume5none
local.edoc.container-issue2none
local.edoc.container-reportyear2020none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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