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2020-05-29Zeitschriftenartikel DOI: 10.25646/7105
PrkA controls peptidoglycan biosynthesisthrough the essential phosphorylation ofReoM
dc.contributor.authorWamp, Sabrina
dc.contributor.authorRutter, Zoe J
dc.contributor.authorRismondo, Jeanine
dc.contributor.authorJennings, Claire E
dc.contributor.authorMöller, Lars
dc.contributor.authorLewis, Richard J
dc.contributor.authorHalbedel, Sven
dc.date.accessioned2020-08-21T12:54:44Z
dc.date.available2020-08-21T12:54:44Z
dc.date.issued2020-05-29none
dc.identifier.other10.7554/eLife.56048
dc.identifier.urihttp://edoc.rki.de/176904/6946
dc.description.abstractPeptidoglycan (PG) is the main component of bacterial cell walls and the target formany antibiotics. PG biosynthesis is tightly coordinated with cell wall growth and turnover, andmany of these control activities depend upon PASTA-domain containing eukaryotic-like serine/threonine protein kinases (PASTA-eSTK) that sense PG fragments. However, only a few PGbiosynthetic enzymes are direct kinase substrates. Here, we identify the conserved ReoM proteinas a novel PASTA-eSTK substrate in the Gram-positive pathogenListeria monocytogenes. Our datashow that the phosphorylation of ReoM is essential as it controls ClpCP-dependent proteolyticdegradation of the essential enzyme MurA, which catalyses the first committed step in PGbiosynthesis. We also identify ReoY as a second novel factor required for degradation of ClpCPsubstrates. Collectively, our data imply that the first committed step of PG biosynthesis is activatedthrough control of ClpCP protease activity in response to signals of PG homeostasis imbalance.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectResearch Articleeng
dc.subjectMicrobiology and Infectious Diseaseeng
dc.subjectPasta-Domaineng
dc.subjectserine/threonine protein kinaseeng
dc.subjectpeptidoglycaneng
dc.subjectprotein degradationeng
dc.subjectClpCP proteaseeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titlePrkA controls peptidoglycan biosynthesisthrough the essential phosphorylation ofReoMnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6946-1
dc.identifier.doihttp://dx.doi.org/10.25646/7105
dc.type.versionpublishedVersionnone
local.edoc.container-titleeLifenone
local.edoc.pages31none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://elifesciences.org/articles/56048none
local.edoc.container-periodicalpart-creatoreLifenone
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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