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2010-10-05Zeitschriftenartikel DOI: 10.1371/journal.pntd.0000833
2nd International External Quality Control Assessment for the Molecular Diagnosis of Dengue Infections
dc.contributor.authorDomingo, Cristina
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorTeichmann, Anette
dc.contributor.authorKaiser, Marco
dc.contributor.authorRumer, Leonid
dc.contributor.authorJarman, Richard G.
dc.contributor.authorMantke, Oliver Donoso
dc.date.accessioned2018-05-07T14:07:48Z
dc.date.available2018-05-07T14:07:48Z
dc.date.created2010-10-19
dc.date.issued2010-10-05none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/relbsMR84lp5A/PDF/25ndcElzXZV7g.pdf
dc.identifier.urihttp://edoc.rki.de/176904/721
dc.description.abstractBackground: Currently dengue viruses (DENV) pose an increasing threat to over 2.5 billion people in over 100 tropical and sub-tropical countries worldwide. International air travel is facilitating rapid global movement of DENV, increasing the risk of severe dengue epidemics by introducing different serotypes. Accurate diagnosis is critical for early initiation of preventive measures. Different reverse transcriptase PCR (RT-PCR) methods are available, which should be evaluated and standardized. Epidemiological and laboratory-based surveillance is required to monitor and guide dengue prevention and control programmes, i.e., by mosquito control or possible vaccination (as soon as an effective and safe vaccine becomes available). Objective: The purpose of the external quality assurance (EQA) study described is to assess the efficiency and accuracy of dengue molecular diagnosis methods applied by expert laboratories. Study Design: A panel of 12 human plasma samples was distributed and tested for DENV-specific RNA. The panel comprised 9 samples spiked with different DENV serotypes (DENV-1 to DENV-4), including 10-fold dilution series of DENV-1 and DENV-3. Two specificity controls consisted of a sample with a pool of 4 other flaviviruses and a sample with chikungunya virus. A negative control sample was also included. Results: Thirty-seven laboratories (from Europe, Middle East Asia, Asia, the Americas/Caribbean, and Africa) participated in this EQA study, and reports including 46 sets of results were returned. Performance among laboratories varied according to methodologies used. Only 5 (10.9%) data sets met all criteria with optimal performance, and 4 (8.7%) with acceptable performance, while 37 (80.4%) reported results showed the need for improvement regarding accomplishment of dengue molecular diagnosis. Failures were mainly due to lack of sensitivity and the presence of false positives. Conclusions: The EQA provides information on each laboratory’s efficacy of RT-PCR techniques for dengue diagnosis and indicates for most laboratories an urgent need to improve sensitivity and specificity.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectHumanseng
dc.subjectQuality Assuranceeng
dc.subjectSensitivity and Specificityeng
dc.subjectReverse Transcriptase Polymerase Chain Reaction/methodseng
dc.subjectEuropeeng
dc.subjectVirology/methodseng
dc.subjectAfricaeng
dc.subjectInternational Cooperationeng
dc.subjectChikungunya virus/geneticseng
dc.subjectChikungunya virus/isolation & purificationeng
dc.subjectDengue Virus/geneticseng
dc.subjectDengue Virus/isolation & purificationeng
dc.subjectDengue/diagnosiseng
dc.subjectRNA Viral/geneticseng
dc.subjectAmericaseng
dc.subjectAsiaeng
dc.subjectMiddle Easteng
dc.subjectPlasma/virologyeng
dc.subjectHealth Care/methodseng
dc.subjectHealth Care/statistics & numerical dataeng
dc.subjectRNA Viral/isolation & purificationeng
dc.subjectReverse Transcriptase Polymerase Chain Reaction/standardseng
dc.subjectVirology/standardseng
dc.subject.ddc610 Medizin
dc.title2nd International External Quality Control Assessment for the Molecular Diagnosis of Dengue Infections
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011056
dc.identifier.doi10.1371/journal.pntd.0000833
dc.identifier.doihttp://dx.doi.org/10.25646/646
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0000833
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume4
local.edoc.container-issue10
local.edoc.container-year2010

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