2010-09-14Zeitschriftenartikel DOI: 10.1186/1743-422X-7-226
Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia
Mendez, Jairo A.
Usme-Ciro, Jose A.
Rey, Gloria J.
Sanchez, Juan A.
Gallego-Gomez, Juan C.
Background: Dengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia, the virus was first detected in the earliest 70′s when the disease became a major public health concern. Since then, all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 (DENV-1), there are not studies about its origin, genetic diversity and distribution. Results: We used 224 bp corresponding to the carboxyl terminus of envelope (E) gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I, likely representing a sole introduction that did not spread. The oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987, a split up generated 2 lineages that have been evolving separately, although not major aminoacid changes in the analyzed region were found. Conclusion: DENV-1 has been circulating since 1978 in Colombia. Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Viral strains used in the present study did not form a monophyletic group, which is evidence of a polyphyletic origin. We report the rapid spread patterns and high evolution rate of the different DENV-1 lineages.
No license information
Show related Items with similar Title, Author, Creator or Subject.
2014-01-23ZeitschriftenartikelAutochthonous dengue virus infection in Japan imported into Germany, September 2013 Schmidt-Chanasit, Jonas; Emmerich, Petra; Tappe, D.; Günther, Stephan; Schmidt, S.; Wolff, D.; Hentschel, K.; Sagebiel, Daniel; Schöneberg, Irene; Stark, Klaus; Frank, ChristinaIn September 2013, dengue virus (DENV) infection was diagnosed in a German traveller returning from Japan. DENV-specific IgM and IgG and DENV NS1 antigen were detected in the patient’s blood, as were DENV serotype 2-specific ...
2006-07-21ZeitschriftenartikelClinical features and pitfalls in the laboratory diagnosis of dengue in travellers Wichmann, Ole; Stark, Klaus; Shu, Pei-Yun; Niedrig, Matthias; Frank, Christina; Huang, Jyh-Hsiung; Jelinek, TomasBackground: Several enzyme-linked immunosorbent assay (ELISA)-kits are commercially available for the rapid diagnosis of dengue infection, and have demonstrated good sensitivity and specificity in paired serum samples. ...
2010-10-07ZeitschriftenartikelDengue virus infection in a traveller returning from Croatia to Germany Schmidt-Chanasit, Jonas; Haditsch, M.; Schöneberg, Irene; Günther, Stephan; Stark, Klaus; Frank, ChristinaDengue virus (DENV) is endemic in south-east Asia and Central to South America. In August 2010, a DENV infection was diagnosed in a German traveller returning from a trip to Croatia in south-east Europe. The patient presented ...