2010-11-16Zeitschriftenartikel DOI: 10.1371/journal.pntd.0000883
Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination.
Klade, Christoph S.
A new commercial anti-Japanese encephalitis virus IgM and IgG indirect immunofluorescence test (IIFT) was evaluated for the detection of the humoral immune response after Japanese encephalitis vaccination. The IgM IIFT was compared to two IgM capture ELISAs and the IgG IIFT was analysed in comparison to a plaque reduction neutralization test (PRNT50) and an IgG ELISA. Moreover, the course of the immune reaction after vaccination with an inactivated JEV vaccine was examined. For the present study 300 serum samples from different blood withdrawals from 100 persons vaccinated against Japanese encephalitis were used. For the IgM evaluation, altogether 78 PRNT50 positive samples taken 7 to 56 days after vaccination and 78 PRNT50 negative sera were analyzed with the Euroimmun anti-JEV IgM IIFT, the Panbio Japanese Encephalitis – Dengue IgM Combo ELISA and the InBios JE Detect IgM capture ELISA. For the IgG evaluation, 100 sera taken 56 days after vaccination and 100 corresponding sera taken before vaccination were tested in the PRNT50, the Euroimmun anti-JEV IgG IIFT, and the InBios JE Detect IgG ELISA. The Euroimmun IgM IIFT showed in comparison to the Panbio ELISA a specificity of 95% and a sensitivity of 86%. With respect to the InBios ELISA, the values were 100% and 83.9%, respectively. The analysis of the Euroimmun IgG IIFT performance and the PRNT50 results demonstrated a specificity of 100% and a sensitivity of 93.8%, whereas it was not possible to detect more than 6.6% of the PRNT50 positive sera as positive with the InBios JE Detect IgG ELISA. Thus, the IIFT is a valuable alternative to the established methods in detecting anti-JEV antibodies after vaccination in travellers and it might prove useful for the diagnosis of acutely infected persons.
No license information
Show related Items with similar Title, Author, Creator or Subject.
2014-01-23ZeitschriftenartikelAutochthonous dengue virus infection in Japan imported into Germany, September 2013 Schmidt-Chanasit, Jonas; Emmerich, Petra; Tappe, D.; Günther, Stephan; Schmidt, S.; Wolff, D.; Hentschel, K.; Sagebiel, Daniel; Schöneberg, Irene; Stark, Klaus; Frank, ChristinaIn September 2013, dengue virus (DENV) infection was diagnosed in a German traveller returning from Japan. DENV-specific IgM and IgG and DENV NS1 antigen were detected in the patient’s blood, as were DENV serotype 2-specific ...
2010-10-07ZeitschriftenartikelDengue virus infection in a traveller returning from Croatia to Germany Schmidt-Chanasit, Jonas; Haditsch, M.; Schöneberg, Irene; Günther, Stephan; Stark, Klaus; Frank, ChristinaDengue virus (DENV) is endemic in south-east Asia and Central to South America. In August 2010, a DENV infection was diagnosed in a German traveller returning from a trip to Croatia in south-east Europe. The patient presented ...
2010-05-24ZeitschriftenartikelInducible costimulator (ICOS) blockade inhibits accumulation of polyfunctional T helper 1/T helper 17 cells and mitigates autoimmune arthritis Frey, Oliver; Meisel, Juliane; Hutloff, Andreas; Bonhagen, Kerstin; Bruns, Lisa; Kroczek, Richard; Morawietz, Lars; Kamradt, ThomasObjectives: Inducible costimulator (ICOS) and its ligand (ICOSL) regulate T and B cell responses. Glucose-6-phosphate isomerase (G6PI)-induced arthritis requires T and B lymphocytes. It was hypothesised that blocking ...