Interferon Beta Modulates Endothelial Damage in Patients with Cardiac Persistence of Human Parvovirus B19 Infection
dc.contributor.author | Schmidt-Lucke, Caroline | |
dc.contributor.author | Spillmann, Frank | |
dc.contributor.author | Bock, Thomas | |
dc.contributor.author | Kühl, Uwe | |
dc.contributor.author | Linthout, Sophie van | |
dc.contributor.author | Schultheiss, Heinz-Peter | |
dc.contributor.author | Tschöpe, Carsten | |
dc.date.accessioned | 2018-05-07T14:32:52Z | |
dc.date.available | 2018-05-07T14:32:52Z | |
dc.date.created | 2011-04-08 | |
dc.date.issued | 2010-03-15 | none |
dc.identifier.other | http://edoc.rki.de/oa/articles/re2hmRrfBqTY/PDF/28rQSi4QT4F8.pdf | |
dc.identifier.uri | http://edoc.rki.de/176904/856 | |
dc.description.abstract | Background: In a phase 1 study, we investigated whether interferon beta reduced endothelial damage in patients with cardiac persistence of human parvovirus B19 (B19V) infection. Methods and results: In vitro, B19V infected cultivated endothelial cells (ECs), which led to a reduction in their viability (Pp.007). Interferon beta suppressed B19V replication by 63% (Pp.008) in ECs and increased their viability (Pp.021). Circulating mature apoptotic ECs (CMAECs [CD45-CD146+ cells expressing von Willebrand factor and annexin V]) and circulating progenitor cells (CPCs [CD34+KDR+ cells]) were quantified by flow cytometry in 9 symptomatic patients with cardiac B19V infection before and after 6 months of interferon beta therapy (16 MU) and were compared to levels in 9 healthy control subjects. Endothelial dysfunction was measured using flow-mediated dilatation of the forearm. Patients with B19V persistence had significantly higher (Pp.004) levels of CMAECs than did control subjects, which normalized after treatment (mean standard deviation, 0.06%-0.08% vs 0.01%-0.006%; Pp.008). Similar improvement was shown for flow-mediated dilatation (Pp.04) in the treatment group only (Pp.017 for the comparison with untreated patients with B19V persistence [np5]). There were significantly higher numbers of CPCs in patients with B19V persistence before therapy (mean standard deviation, 0.04%-0.05% vs 0.01%-0.004%; Pp.02) than in control subjects, which normalized after treatment (Pp.03). Conclusion: Thus, we present (for the first time, to our knowledge) a modulation of virally induced chronic endothelial damage specifically, EC apoptosis and endothelial regeneration. | eng |
dc.language.iso | eng | |
dc.publisher | Robert Koch-Institut, Infektionskrankheiten / Erreger | |
dc.subject | Adolescent | eng |
dc.subject | Cell Line | eng |
dc.subject | Humans | eng |
dc.subject | Female | eng |
dc.subject | Male | eng |
dc.subject | Middle Aged | eng |
dc.subject | Risk Factors | eng |
dc.subject | Adult | eng |
dc.subject | Aged | eng |
dc.subject | Treatment Outcome | eng |
dc.subject | Young Adult | eng |
dc.subject | Antiviral Agents/pharmacology | eng |
dc.subject | Antiviral Agents/therapeutic use | eng |
dc.subject | Apoptosis/drug effects | eng |
dc.subject | Cardiovascular Diseases/prevention & control | eng |
dc.subject | Cardiovascular Diseases/virology | eng |
dc.subject | Endothelial Cells/drug effects | eng |
dc.subject | Endothelial Cells/pathology | eng |
dc.subject | Endothelial Cells/virology | eng |
dc.subject | Endothelium | eng |
dc.subject | Vascular/drug effects | eng |
dc.subject | Vascular/pathology | eng |
dc.subject | Endothelium Vascular/virology | eng |
dc.subject | Flow Cytometry | eng |
dc.subject | Interferon-beta/pharmacology | eng |
dc.subject | Interferon-beta/therapeutic use | eng |
dc.subject | Parvoviridae Infections/complications | eng |
dc.subject | Parvoviridae Infections/drug therapy* | eng |
dc.subject | Parvovirus B19 | eng |
dc.subject | Human/drug effects | eng |
dc.subject.ddc | 610 Medizin | |
dc.title | Interferon Beta Modulates Endothelial Damage in Patients with Cardiac Persistence of Human Parvovirus B19 Infection | |
dc.type | periodicalPart | |
dc.identifier.urn | urn:nbn:de:0257-10013927 | |
dc.identifier.doi | 10.1086/650700 | |
dc.identifier.doi | http://dx.doi.org/10.25646/781 | |
local.edoc.container-title | Journal of Infectious Diseases | |
local.edoc.container-text | Schmidt-Lucke, C., Spillmann, F., Bock, T., Kühl, U., Van Linthout, S., Schultheiss, H.-P., Tschöpe, C. Interferon beta modulates endothelial damage in patients with cardiac persistence of human parvovirus B19 infection (2010) Journal of Infectious Diseases, 201 (6), pp. 936-945. | |
local.edoc.fp-subtype | Artikel | |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
local.edoc.container-url | http://jid.oxfordjournals.org/content/201/6/936.long | |
local.edoc.container-publisher-name | University of Chicago Press | |
local.edoc.container-volume | 201 | |
local.edoc.container-issue | 6 | |
local.edoc.container-year | 2010 |