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2011-07-07Zeitschriftenartikel DOI: 10.1371/journal.ppat.1002117
Unique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis.
dc.contributor.authorUliczka, Frank
dc.contributor.authorPisano, Fabio
dc.contributor.authorSchaake, Julia
dc.contributor.authorStolz, Tatjana
dc.contributor.authorRohde, Manfred
dc.contributor.authorFruth, Angelika
dc.contributor.authorStrauch, Eckhard
dc.contributor.authorSkurnik, Mikael
dc.contributor.authorBatzilla, Julia
dc.contributor.authorRakin, Alexander
dc.contributor.authorHeesemann, Jürgen
dc.contributor.authorDersch, Petra
dc.date.accessioned2018-05-07T14:45:53Z
dc.date.available2018-05-07T14:45:53Z
dc.date.created2011-09-01
dc.date.issued2011-07-07none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reeQGIo7WUhm/PDF/247mpj5mInEcs.pdf
dc.identifier.urihttp://edoc.rki.de/176904/926
dc.description.abstractMany enteric pathogens are equipped with multiple cell adhesion factors which are important for host tissue colonization and virulence. Y. enterocolitica, a common food-borne pathogen with invasive properties, uses the surface proteins invasin and YadA for host cell binding and entry. In this study, we demonstrate unique cell adhesion and invasion properties of Y. enterocolitica serotype O:3 strains, the most frequent cause of human yersiniosis, and show that these differences are mainly attributable to variations affecting the function and expression of invasin in response to temperature. In contrast to other enteric Yersinia strains, invasin production in O:3 strains is constitutive and largely enhanced compared to other Y. enterocolitica serotypes, in which invA expression is temperature-regulated and significantly reduced at 37°C. Increase of invasin levels is caused by (i) an IS1667 insertion into the invA promoter region, which includes an additional promoter and RovA and H-NS binding sites, and (ii) a P98S substitution in the invA activator protein RovA rendering the regulator less susceptible to proteolysis. Both variations were shown to influence bacterial colonization in a murine infection model. Furthermore, we found that co-expression of YadA and down-regulation of the O-antigen at 37°C is required to allow efficient internalization by the InvA protein. We conclude that even small variations in the expression of virulence factors can provoke a major difference in the virulence properties of closely related pathogens which may confer better survival or a higher pathogenic potential in a certain host or host environment.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectBacterial/geneticseng
dc.subjectHumanseng
dc.subjectFemaleeng
dc.subjectAnimalseng
dc.subjectMiceeng
dc.subjectYersinia enterocolitica/physiologyeng
dc.subjectVirulence Factors/geneticseng
dc.subjectMice Inbred BALB Ceng
dc.subjectHot Temperatureeng
dc.subjectDisease Models Animaleng
dc.subjectHost-Pathogen Interactionseng
dc.subjectCells Culturedeng
dc.subjectAdaptation Physiologicaleng
dc.subjectAdhesinseng
dc.subjectAdhesins Bacterial/metabolismeng
dc.subjectBacterial Adhesion/physiologyeng
dc.subjectDown-Regulationeng
dc.subjectGene Expression Regulation Bacterialeng
dc.subjectO Antigens/geneticseng
dc.subjectO Antigens/metabolismeng
dc.subjectVirulence Factors/metabolismeng
dc.subjectYersinia Infectionseng
dc.subjectYersinia enterocolitica/pathogenicityeng
dc.subject.ddc610 Medizin
dc.titleUnique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10015101
dc.identifier.doi10.1371/journal.ppat.1002117
dc.identifier.doihttp://dx.doi.org/10.25646/851
local.edoc.container-titlePLoS Pathogens
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1002117
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue7
local.edoc.container-year2011

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