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2021-04-16Zeitschriftenartikel
Frequency, Local Dynamics, and Genomic Characteristics of ESBL-Producing Escherichia coli Isolated From Specimens of Hospitalized Horses
dc.contributor.authorKauter, Anne
dc.contributor.authorEpping, Lennard
dc.contributor.authorGhazisaeed, Fereshteh
dc.contributor.authorLübke-Becker, Antina
dc.contributor.authorWolf, Silver A.
dc.contributor.authorKannapin, Dania
dc.contributor.authorStoeckle, Sabita D.
dc.contributor.authorSemmler, Torsten
dc.contributor.authorGünther, Sebastian
dc.contributor.authorGehlen, Heidrun
dc.contributor.authorWalther, Birgit
dc.date.accessioned2022-02-01T09:41:13Z
dc.date.available2022-02-01T09:41:13Z
dc.date.issued2021-04-16none
dc.identifier.other10.3389/fmicb.2021.671676
dc.identifier.urihttp://edoc.rki.de/176904/9309
dc.description.abstractPrevious research identified veterinary clinics as hotspots with respect to accumulation and spread of multidrug resistant extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (EC). Therefore, promoting the prudent use of antibiotics to decrease selective pressure in that particular clinical environment is preferable to enhance biosecurity for animal patients and hospital staff. Accordingly, this study comparatively investigated the impact of two distinct perioperative antibiotic prophylaxis (PAP) regimens (short-term versus prolonged) on ESBL-EC carriage of horses subjected to colic surgery. While all horses received a combination of penicillin/gentamicin (P/G) as PAP, they were assigned to either the “single-shot group” (SSG) or the conventional “5-day group” (5DG). Fecal samples collected on arrival (t0), on the 3rd (t1) and on the 10th day after surgery (t2) were screened for ESBL-EC. All isolates were further investigated using whole genome sequences. In total, 81 of 98 horses met the inclusion criteria for this study. ESBL-EC identified in samples available at t0, t1 and t2 were 4.8% (SSG) and 9.7% (5DG), 37% (SSG) and 47.2% (5DG) as well as 55.6% (SSG) and 56.8% (5DG), respectively. Regardless of the P/G PAP regimen, horses were 9.12 times (95% CI 2.79–29.7) more likely to carry ESBL-EC at t1 compared to t0 (p < 0.001) and 15.64 times (95% CI 4.57–53.55) more likely to carry ESBL-EC at t2 compared to t0 (p < 0.001). ESBL-EC belonging to sequence type (ST) 10, ST86, ST641, and ST410 were the most prevalent lineages, with blaCTX–M–1 (60%) being the dominant ESBL gene. A close spatio-temporal relationship between isolates sharing a particular ST was revealed by genome analysis, strongly indicating local spread. Consequently, hospitalization itself has a strong impact on ESBL-EC isolation rates in horses, possibly masking differences between distinct PAP regimens. The results of this study reveal accumulation and spread of multi-drug resistant ESBL-EC among horses subjected to colic surgery with different P/G PAP regimens, challenging the local hygiene management system and work-place safety of veterinary staff. Moreover, the predominance of particular ESBL-EC lineages in clinics providing health care for horses needs further investigation.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectESBLeng
dc.subjecthorseeng
dc.subjectEscherichia colieng
dc.subjectantibiotic resistanceeng
dc.subjectmultidrug resistanteng
dc.subjectspreadeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleFrequency, Local Dynamics, and Genomic Characteristics of ESBL-Producing Escherichia coli Isolated From Specimens of Hospitalized Horsesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9309-7
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicrobiologynone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.671676/fullnone
local.edoc.container-publisher-nameFrontiersnone
local.edoc.container-volume12none
local.edoc.container-year2021none
local.edoc.container-firstpage1none
local.edoc.container-lastpage13none
local.edoc.rki-departmentZentrum für Biologische Gefahren und Spezielle Pathogenenone
dc.description.versionPeer Reviewednone

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