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2021-08-20Zeitschriftenartikel
Propagation of CJD Prions in Primary Murine Glia Cells Expressing Human PrPc
dc.contributor.authorWälzlein, Joo-Hee
dc.contributor.authorSchwenke, Karla A.
dc.contributor.authorBeekes, Michael
dc.date.accessioned2022-02-02T09:11:23Z
dc.date.available2022-02-02T09:11:23Z
dc.date.issued2021-08-20none
dc.identifier.other10.3390/pathogens10081060
dc.identifier.urihttp://edoc.rki.de/176904/9334
dc.description.abstractThere are various existing cell models for the propagation of animal prions. However, in vitro propagation of human prions has been a long-standing challenge. This study presents the establishment of a long-term primary murine glia culture expressing the human prion protein homozygous for methionine at codon 129, which allows in vitro propagation of Creutzfeldt–Jakob disease (CJD) prions (variant CJD (vCJD) and sporadic CJD (sCJD) type MM2). Prion propagation could be detected by Western blotting of pathological proteinase K-resistant prion protein (PrPSc) from 120 days post exposure. The accumulation of PrPSc could be intensified by adding a cationic lipid mixture to the infectious brain homogenate at the time of infection. Stable propagation of human prions in a long-term murine glia cell culture represents a new tool for future drug development and for mechanistic studies in the field of human prion biology. In addition, our cell model can reduce the need for bioassays with human prions and thereby contributes to further implementation of the 3R principles aiming at replacement, reduction and refinement of animal experiments.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectprionseng
dc.subjectCreutzfeldt–Jakob diseaseeng
dc.subjecthuman cell modeleng
dc.subjectgliaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titlePropagation of CJD Prions in Primary Murine Glia Cells Expressing Human PrPcnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9334-0
dc.type.versionpublishedVersionnone
local.edoc.container-titlePathogensnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/2076-0817/10/8/1060none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume10none
local.edoc.container-issue8none
local.edoc.container-year2021none
local.edoc.container-firstpage1none
local.edoc.container-lastpage8none
local.edoc.rki-departmentZentrum für Biologische Gefahren und Spezielle Pathogenenone
dc.description.versionPeer Reviewednone

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