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2022-02-02Zeitschriftenartikel
Advanced sequencing approaches detected insertions of viral and human origin in the viral genome of chronic hepatitis E virus patients
dc.contributor.authorPapp, C.‑Patrick
dc.contributor.authorBiedermann, Paula
dc.contributor.authorHarms, Dominik
dc.contributor.authorWang, Bo
dc.contributor.authorKebelmann, Marianne
dc.contributor.authorChoi, Mira
dc.contributor.authorHelmuth, Johannes
dc.contributor.authorCorman, Victor M.
dc.contributor.authorThürmer, Andrea
dc.contributor.authorAltmann, Britta
dc.contributor.authorKlink, Patrycja
dc.contributor.authorHofmann, Jörg
dc.contributor.authorBock, C.‑Thomas
dc.date.accessioned2022-03-17T07:19:38Z
dc.date.available2022-03-17T07:19:38Z
dc.date.issued2022-02-02none
dc.identifier.other10.1038/s41598-022-05706-w
dc.identifier.urihttp://edoc.rki.de/176904/9511
dc.description.abstractThe awareness of hepatitis E virus (HEV) increased significantly in the last decade due to its unexpectedly high prevalence in high-income countries. There, infections with HEV-genotype 3 (HEV-3) are predominant which can progress to chronicity in immunocompromised individuals. Persistent infection and antiviral therapy can select HEV-3 variants; however, the spectrum and occurrence of HEV-3 variants is underreported. To gain in-depth insights into the viral population and to perform detailed characterization of viral genomes, we used a new approach combining long-range PCR with next-generation and third-generation sequencing which allowed near full-length sequencing of HEV-3 genomes. Furthermore, we developed a targeted ultra-deep sequencing approach to assess the dynamics of clinically relevant mutations in the RdRp-region and to detect insertions in the HVR-domain in the HEV genomes. Using this new approach, we not only identified several insertions of human (AHNAK, RPL18) and viral origin (RdRp-derived) in the HVR-region isolated from an exemplary sample but detected a variant containing two different insertions simultaneously (AHNAK- and RdRp-derived). This finding is the first HEV-variant recognized as such showing various insertions in the HVR-domain. Thus, this molecular approach will add incrementally to our current knowledge of the HEV-genome organization and pathogenesis in chronic hepatitis E.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleAdvanced sequencing approaches detected insertions of viral and human origin in the viral genome of chronic hepatitis E virus patientsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9511-5
dc.type.versionpublishedVersionnone
local.edoc.container-titleScientifc Reportsnone
local.edoc.container-issn2045-2322none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41598-022-05706-wnone
local.edoc.container-publisher-nameNature Researchnone
local.edoc.container-volume12none
local.edoc.container-issue1720none
local.edoc.container-year2022none
dc.description.versionPeer Reviewednone

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