2011-09-08Zeitschriftenartikel DOI: 10.1371/journal.pone.0024360
Rare Occurrence of Methicillin-Resistant Staphylococcus aureus CC130 with a Novel mecA Homologue in Humans in Germany
MRSA CC130 containing the mecA homologue mecALGA251 were reported from the UK and from Denmark so far from cattle and humans. Here we report on 11 MRSA CC130 among a sample of 12691 isolates of human origin collected from January 2006 until June 2011. MRSA CC130 grew insufficiently on chromogernic agar plates for detection of MRSA; the agglutination test for presence of PBP2a was negative. We designed primers for specific detection of mecALGA251 as well as for concomitant detection of both, mecLGA251 and mecA. As already described, the isolates exhibited spa-types t843, t1736, and t1773. The ccrA homologue indicated the presence SCCmecXI. When subjected to further characterization by means of a commercially available microarray the isolates were negative for sak chp, and scn, and as expected positive for hla, untruncated hlb, and hld. They furthermore contained edinB, aur, slpA, slpB, slpE. From genes coding for surface and cell wall associated products the ica-operon, cap8, clfA, clfF, ebpS, fnbA, fnbB, sdrC were detected but not cna. The isolates were negative for enterotoxin genes and tst, as well as for eta, and etb; agr-type was III.
Dateien zu dieser Publikation
Anzeige der Publikationen mit ähnlichem Titel, Autor, Urheber und Thema.
2009-08-06ZeitschriftenartikelTranscription of the phage-encoded Panton–Valentine leukocidin of Staphylococcus aureus is dependent on the phage life-cycle and on the host background Wirtz, Christiane; Witte, Wolfgang; Wolz, Christiane; Goerke, ChristianePanton-Valentine leukocidin (PVL) is a pore-forming, bi-component toxin secreted by Staphylococcus aureus strains epidemiologically associated with diseases such as necrotizing pneumonia and skin and soft-tissue infections. ...
2009-08-24ZeitschriftenartikelPrevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among pigs on German farms and import of livestock-related MRSA into hospitals Köck, Robin; Harlizius, J.; Bressan, N.; Laerberg, R.; Wieler, Lothar H.; Witte, Wolfgang; Deurenberg, R. H.; Voss, A.; Becker, Karsten; Friedrich, Alexander W.The aim of this study was to evaluate the prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among pigs and estimate the impact of this animal reservoir on human healthcare. Nasal ...
2013-01-08ZeitschriftenartikelA genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic Holden, Matthew T.G.; Hsu, Li-Yang; Kurt, Kevin; Weinert, Lucy A.; Mather, Alison E.; Harris, Simon R.; Strommenger, Birgit; Layer, Franziska; Witte, Wolfgang; Lencastre, Herminia de; Skov, Robert; Westh, Henrik; Zemličková, Helena; Coombs, Geoffrey; Kearns, Angela M.; Hill, Robert L.R.; Edgeworth, Jonathan; Gould, Ian; Gant, Vanya; Cooke, Jonathan; Edwards, Giles F.; McAdam, Paul R.; Templeton, Kate E.; McCann, Angela; Nübel, UlrichThe widespread use of antibiotics in association with high-density clinical care has driven the emergence of drug-resistant bacteria that are adapted to thrive in hospitalized patients. Of particular concern are globally ...