Zur Kurzanzeige

2022-03-28Zeitschriftenartikel
Short- and long-range interactions in the HIV-1 5′ UTR regulate genome dimerization and packaging
dc.contributor.authorYe, Liqing
dc.contributor.authorGribling-Burrer, Anne-Sophie
dc.contributor.authorBohn, Patrick
dc.contributor.authorKibe, Anuja
dc.contributor.authorBörtlein, Charlene
dc.contributor.authorAmbi, Uddhav B.
dc.contributor.authorAhmad, Shazeb
dc.contributor.authorOlguin-Nava, Marco
dc.contributor.authorSmith, Maureen
dc.contributor.authorCaliskan, Neva
dc.contributor.authorvon Kleist, Max
dc.contributor.authorSmyth, Redmond P.
dc.date.accessioned2022-06-08T06:48:17Z
dc.date.available2022-06-08T06:48:17Z
dc.date.issued2022-03-28none
dc.identifier.other10.1038/s41594-022-00746-2
dc.identifier.urihttp://edoc.rki.de/176904/9792
dc.description.abstractRNA dimerization is the noncovalent association of two human immunodeficiency virus-1 (HIV-1) genomes. It is a conserved step in the HIV-1 life cycle and assumed to be a prerequisite for binding to the viral structural protein Pr55Gag during genome packaging. Here, we developed functional analysis of RNA structure-sequencing (FARS-seq) to comprehensively identify sequences and structures within the HIV-1 5′ untranslated region (UTR) that regulate this critical step. Using FARS-seq, we found nucleotides important for dimerization throughout the HIV-1 5′ UTR and identified distinct structural conformations in monomeric and dimeric RNA. In the dimeric RNA, key functional domains, such as stem-loop 1 (SL1), polyadenylation signal (polyA) and primer binding site (PBS), folded into independent structural motifs. In the monomeric RNA, SL1 was reconfigured into long- and short-range base pairings with polyA and PBS, respectively. We show that these interactions disrupt genome packaging, and additionally show that the PBS–SL1 interaction unexpectedly couples the PBS with dimerization and Pr55Gag binding. Altogether, our data provide insights into late stages of HIV-1 life cycle and a mechanistic explanation for the link between RNA dimerization and packaging.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectRiboswitcheseng
dc.subjectRNAeng
dc.subjectStructure determinationeng
dc.subjectVirologyeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleShort- and long-range interactions in the HIV-1 5′ UTR regulate genome dimerization and packagingnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9792-4
dc.type.versionpublishedVersionnone
local.edoc.container-titleNature Structural & Molecular Biologynone
local.edoc.container-issn1545-9985none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41594-022-00746-2none
local.edoc.container-publisher-nameNature Publishing Groupnone
local.edoc.container-volume29none
local.edoc.container-year2022none
local.edoc.container-firstpage306none
local.edoc.container-lastpage319none
dc.description.versionPeer Reviewednone

Zur Kurzanzeige