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2011-10-12Zeitschriftenartikel DOI: 10.1371/journal.pone.0025592
No Detection of XMRV in Blood Samples and Tissue Sections from Prostate Cancer Patients in Northern Europe.
dc.contributor.authorStieler, Kristin
dc.contributor.authorSchindler, Sarah
dc.contributor.authorSchlomm, Thorsten
dc.contributor.authorHohn, Oliver
dc.contributor.authorBannert, Norbert
dc.contributor.authorSimon, Ronald
dc.contributor.authorMinner, Sarah
dc.contributor.authorSchindler, Michael
dc.contributor.authorFischer, Nicole
dc.date.accessioned2018-05-07T14:55:45Z
dc.date.available2018-05-07T14:55:45Z
dc.date.created2011-10-27
dc.date.issued2011-10-12none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reubCytl45yzk/PDF/27XbnfNtzVs46.pdf
dc.identifier.urihttp://edoc.rki.de/176904/980
dc.description.abstractBackground: We recently published the rare detection of xenotropic murine leukemia virus-related virus (XMRV) (1/105) in prostate cancer (PCA) tissue of patients in Northern Europe by PCR. The controversial discussion about the virus being detected in PCA tissue, blood samples from patients suffering from chronic fatigue syndrome (CFS), as well as from a significant number of healthy controls prompted us to deepen our studies about detection of XMRV infection applying different detection methods (PCR, cocultivation and immunohistochemistry [IHC]). Methodology/Principal Findings: Peripheral blood mononuclear cells (PBMCs) from 92 PCA and 7 healthy controls were isolated, PHA activated and cocultivated with LNCaP cells for up to 8 weeks. Supernatant of these cells was applied to a reporter cell line, DERSE-iGFP. Furthermore, the PBMCs and cocultivated LNCaP cells were tested for the presence of XMRV by PCR as well as Western Blot analysis. While all PCR amplifications and Western Blot analyses were negative for signs of XMRV infection, DERSE-iGFP cells displayed isolated GFP positive cells in three cases. In all three cases XMRV presence could not be confirmed by PCR technology. In addition, we performed XMRV specific IHC on PCA tissue sections. Whole tissue sections (n = 20), as well as tissue microarrays (TMA) including 50 benign prostate hyperplasia (BPH), 50 low grade and 50 high grade PCA sections and TMAs including breast cancer, colon cancer and normal tissues were stained with two XMRV specific antisera. XMRV protein expression was not detected in any cancer sections included. One BPH tissue displayed XMRV specific protein expression in random isolated basal cells. Conclusion: We were unable to conclusively detect XMRV in the blood from PCA patients or from healthy controls and there is no conclusive evidence of XMRV protein expression in PCA, breast cancer and colon cancer tissue sections tested by IHC staining.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionsepidemiologie
dc.subjectAdulteng
dc.subjectHumanseng
dc.subjectPolymerase Chain Reactioneng
dc.subjectMaleeng
dc.subjectMiddle Agedeng
dc.subjectViral Proteins/metabolismeng
dc.subjectVirus Replicationeng
dc.subjectCase-Control Studieseng
dc.subjectAgedeng
dc.subjectEuropeeng
dc.subjectProstatic Neoplasms/virologyeng
dc.subjectRetroviridae Infections/virologyeng
dc.subjectCell Line Tumoreng
dc.subjectLeukocytes Mononuclear/virologyeng
dc.subjectXenotropic murine leukemia virus-related virus/geneticseng
dc.subjectCell Separationeng
dc.subjectCoculture Techniqueseng
dc.subjectImmunohistochemistryeng
dc.subjectLymphocyte Activation/immunologyeng
dc.subjectProstate/pathologyeng
dc.subjectProstate/virologyeng
dc.subjectProstatic Neoplasms/bloodeng
dc.subjectProstatic Neoplasms/pathologyeng
dc.subjectXenotropic murine leukemia virus-related virus/isolation & purificationeng
dc.subjectXenotropic murine leukemia virus-related virus/physiologyeng
dc.subject.ddc610 Medizin
dc.titleNo Detection of XMRV in Blood Samples and Tissue Sections from Prostate Cancer Patients in Northern Europe.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10015869
dc.identifier.doi10.1371/journal.pone.0025592
dc.identifier.doihttp://dx.doi.org/10.25646/905
local.edoc.container-titlePLoS ONE
local.edoc.container-textStieler K, Schindler S, Schlomm T, Hohn O, Bannert N, et al. (2011) No Detection of XMRV in Blood Samples and Tissue Sections from Prostate Cancer Patients in Northern Europe. PLoS ONE 6(10): e25592.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0025592
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume6
local.edoc.container-issue10
local.edoc.container-year2011

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