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2012-03-07Zeitschriftenartikel DOI: 10.1007/s00216-012-5767-3
De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics
dc.contributor.authorKalb, Suzanne R.
dc.contributor.authorBaudys, Jakub
dc.contributor.authorRees, Jon C.
dc.contributor.authorSmith, Theresa J.
dc.contributor.authorSmith, Leonard A.
dc.contributor.authorHelma, Charles H.
dc.contributor.authorHill, Karen
dc.contributor.authorKull, Skadi
dc.contributor.authorKirchner, Sebastian
dc.contributor.authorDorner, Martin
dc.contributor.authorDorner, Brigitte
dc.contributor.authorPirkle, James L.
dc.contributor.authorBarr, John R.
dc.date.accessioned2018-05-07T15:33:06Z
dc.date.available2018-05-07T15:33:06Z
dc.date.created2012-04-13
dc.date.issued2012-03-07none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reqbgNWipUXpc/PDF/20BWmx0SwpnXE.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1184
dc.description.abstractBotulinum neurotoxins (BoNTs) cause the disease botulism, which can be lethal if untreated. There are seven known serotypes of BoNT, A–G, defined by their response to antisera. Many serotypes are distinguished into differing subtypes based on amino acid sequence, and many subtypes are further differentiated into toxin variants. Previous work in our laboratory described the use of a proteomics approach to distinguish subtype BoNT/A1 from BoNT/A2 where BoNT identities were confirmed after searching data against a database containing protein sequences of all known BoNT/A subtypes. We now describe here a similar approach to differentiate subtypes BoNT/B1, /B2, /B3, /B4, and /B5. Additionally, to identify new subtypes or hitherto unpublished amino acid substitutions, we created an amino acid substitution database covering every possible amino acid change. We used this database to differentiate multiple toxin variants within subtypes of BoNT/B1 and B2. More importantly, with our amino acid substitution database, we were able to identify a novel BoNT/B subtype, designated here as BoNT/B7. These techniques allow for subtype and strain level identification of both known and unknown BoNT/B rapidly with no DNA required.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectBotulinum neurotoxineng
dc.subjectBotulismeng
dc.subjectMass spectrometryeng
dc.subjectProteomicseng
dc.subject.ddc610 Medizin
dc.titleDe novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10023396
dc.identifier.doi10.1007/s00216-012-5767-3
dc.identifier.doihttp://dx.doi.org/10.25646/1109
local.edoc.container-titleAnalytical and Bioanalytical Chemistry
local.edoc.container-textKalb, S.R., Baudys, J., Rees, J.C., Smith, T.J., Smith, L.A., Helma, C.H., Hill, K., Kull, S., Kirchner, S., Dorner, M.B., Dorner, B.G., Pirkle, J.L., Barr, J.R. De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics (2012) Analytical and Bioanalytical Chemistry, 403 (1), pp. 215-226.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.springerlink.com/content/712545031410l701/export-citation/
local.edoc.container-publisher-nameSpringer
local.edoc.container-volume403
local.edoc.container-issue1
local.edoc.container-year2012

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