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2022-06-03Zeitschriftenartikel
Tolerance of biofilm of a carbapenem-resistant Klebsiella pneumoniae involved in a duodenoscopy-associated outbreak to the disinfectant used in reprocessing
dc.contributor.authorBrunke, Melanie S.
dc.contributor.authorKonrat, Katharina
dc.contributor.authorSchaudinn, Christoph
dc.contributor.authorPiening, Brar
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorBecker, Laura
dc.contributor.authorSchwebke, Ingeborg
dc.contributor.authorArvand, Mardjan
dc.date.accessioned2024-09-19T15:28:17Z
dc.date.available2024-09-19T15:28:17Z
dc.date.issued2022-06-03none
dc.identifier.other10.1186/s13756-022-01112-z
dc.identifier.urihttp://edoc.rki.de/176904/12244
dc.description.abstractBackground One possible transmission route for nosocomial pathogens is contaminated medical devices. Formation of biofilms can exacerbate the problem. We report on a carbapenemase-producing Klebsiella pneumoniae that had caused an outbreak linked to contaminated duodenoscopes. To determine whether increased tolerance to disinfectants may have contributed to the outbreak, we investigated the susceptibility of the outbreak strain to disinfectants commonly used for duodenoscope reprocessing. Disinfection efficacy was tested on planktonic bacteria and on biofilm. Methods Disinfectant efficacy testing was performed for planktonic bacteria according to EN standards 13727 and 14561 and for biofilm using the Bead Assay for Biofilms. Disinfection was defined as ≥ 5log10 reduction in recoverable colony forming units (CFU). Results The outbreak strain was an OXA-48 carbapenemase-producing K. pneumoniae of sequence type 101. We found a slightly increased tolerance of the outbreak strain in planktonic form to peracetic acid (PAA), but not to other disinfectants tested. Since PAA was the disinfectant used for duodenoscope reprocessing, we investigated the effect of PAA on biofilm of the outbreak strain. Remarkably, disinfection of biofilm of the outbreak strain could not be achieved by the standard PAA concentration used for duodenoscope reprocessing at the time of outbreak. An increased tolerance to PAA was not observed in a K. pneumoniae type strain tested in parallel. Conclusions Biofilm of the K. pneumoniae outbreak strain was tolerant to standard disinfection during duodenoscope reprocessing. This study establishes for the first time a direct link between biofilm formation, increased tolerance to disinfectants, reprocessing failure of duodenoscopes and nosocomial transmission of carbapenem-resistant K. pneumoniae.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectoutbreakeng
dc.subjectantimicrobial resistanceeng
dc.subjectdisinfectioneng
dc.subjectperacetic acideng
dc.subjectduodenoscopeeng
dc.subjectreprocessingeng
dc.subjectgram-negativeeng
dc.subjectcarbapenemaseeng
dc.subjectOXA-48eng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleTolerance of biofilm of a carbapenem-resistant Klebsiella pneumoniae involved in a duodenoscopy-associated outbreak to the disinfectant used in reprocessingnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12244-0
dc.type.versionpublishedVersionnone
local.edoc.container-titleAntimicrobial Resistance & Infection Controlnone
local.edoc.container-issn2047-2994none
local.edoc.pages7none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://aricjournal.biomedcentral.com/none
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume11none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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