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2010-04-01Zeitschriftenartikel DOI: 10.25646/1248
Characterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/C.
dc.contributor.authorKarlas, Alexander
dc.contributor.authorIrgang, Markus
dc.contributor.authorVotteler, Jörg
dc.contributor.authorSpecke, Volker
dc.contributor.authorOzel, Mushin
dc.contributor.authorKurth, Reinhard
dc.contributor.authorDenner, Joachim
dc.date.accessioned2018-05-07T15:58:31Z
dc.date.available2018-05-07T15:58:31Z
dc.date.created2012-10-02
dc.date.issued2010-04-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reoXiNcHzrYS6/PDF/26Ixg9iHEi4nY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1323
dc.description.abstractBackground: Porcine endogenous retroviruses (PERVs) pose a potential risk for xenotransplantation using pig cells, tissues or organs. A special threat comes from viruses generated by recombination between human-tropic PERV-A and ecotropic PERV-C. Serial passages of a recombinant PERV-A/C on human 293 cells resulted in increased infectious titers and a multimerization of transcription factor binding sites in the viral long terminal repeat (LTR). In contrast to the LTR, the sequence of the env gene did not change, indicating that the LTR represents the determinant of high infectivity. Material/Methods: The virus was further propagated on human cells and characterized by different methods (titration, sequencing, infection experiments, electron microscopy). Results: Further propagation on human 293 cells resulted in deletions and mutations in the LTR. In contrast to low-titer viruses, the high-titer virus was infectious for cells from non-human primates including chimpanzees. Scanning electron microscopy revealed clustering of budding virions at the cell surface of infected human cells and transmission electron microscopy indicated that the virus infects them via receptor-mediated endocytosis. Conclusions: After propagation of PERV on human cells without selection pressure, viruses with different LTR were generated. High titer PERV was shown to infect cells from non-human primates. The experiments performed here simulate the situation in vivo and give an extended characterization of human cell-adapted PERVs.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAnimalseng
dc.subjectCell Lineeng
dc.subjectHumanseng
dc.subjectMolecular Sequence Dataeng
dc.subjectBase Sequenceeng
dc.subjectMacaca mulattaeng
dc.subjectEndogenous Retroviruses/geneticseng
dc.subjectEndogenous Retroviruses/pathogenicityeng
dc.subjectSwine/virologyeng
dc.subjectSpecies Specificityeng
dc.subjectPan troglodyteseng
dc.subjectRNA Splicingeng
dc.subjectTerminal Repeat Sequenceseng
dc.subjectRNA Viral/geneticseng
dc.subjectDNA Viral/geneticseng
dc.subjectVirus Cultivationeng
dc.subjectRecombination Geneticeng
dc.subjectMicroscopy Electron Scanningeng
dc.subjectEndogenous Retroviruses/classificationeng
dc.subjectEndogenous Retroviruses/ultrastructureeng
dc.subjectSequence Homology Nucleic Acideng
dc.subject.ddc610 Medizin
dc.titleCharacterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/C.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10027413
dc.identifier.doihttp://dx.doi.org/10.25646/1248
local.edoc.container-titleAnnals of Transplantation
local.edoc.container-textAlexander Karlas, Markus Irgang, Jörg Votteler, Volker Specke, Mushin Özel, Reinhard Kurth, Joachim Denner. Characterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/C (2010) Annals of Transplantation, 15(2) pp. 45-54.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.annalsoftransplantation.com/index.php?/archives/article/880940
local.edoc.container-publisher-nameSpringer
local.edoc.container-volume15
local.edoc.container-issue2
local.edoc.container-year2010

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