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2011-11-09Zeitschriftenartikel DOI: 10.1016/j.antiviral.2011.10.023
RNA interference inhibits replication of tick-borne encephalitis virus in vitro.
dc.contributor.authorAchazi, Katharina
dc.contributor.authorPatel, Pranav
dc.contributor.authorPaliwal, Ravish
dc.contributor.authorRadonić, Aleksandar
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorMantke, Oliver Donoso
dc.date.accessioned2018-05-07T16:04:27Z
dc.date.available2018-05-07T16:04:27Z
dc.date.created2012-11-09
dc.date.issued2011-11-09none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reXlE7BXTWOkQ/PDF/29L0cnk31JmQ.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1355
dc.description.abstractEach year, up to 10,000 cases of infections with the flavivirus tick-borne encephalitis (TBE) virus that affect the central nervous system are reported in Europe and Asia. Due to the potentially severe adverse effects of post-exposure prophylaxis with TBE virus hyperimmunoglobulin, TBE can currently only be treated symptomatically. An RNA interference (RNAi) approach to inhibit TBE virus replication was therefore developed. In this study we demonstrate for the first time that small interfering RNAs (siRNAs) targeted at the TBE virus genome reduce the quantity of infectious TBE virus particles, TBE virus genome, and TBE virus protein in vitro by up to 85%. The 50% inhibitory dose (DI(50)) of the shRNA plasmid was only 0.05μg/ml. As RNAi-based therapeutics for other diseases are already being evaluated in phases II and III clinical trials, it is possible that RNAi could become valuable tool for controlling TBE virus infection.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectFlaviviruseng
dc.subjectTick-borneencephalitisviruseng
dc.subjectTick-borneencephalitiseng
dc.subjectRNAinterferenceeng
dc.subjectSmall interfering RNAeng
dc.subjectAntiviral drugeng
dc.subject.ddc610 Medizin
dc.titleRNA interference inhibits replication of tick-borne encephalitis virus in vitro.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10028383
dc.identifier.doi10.1016/j.antiviral.2011.10.023
dc.identifier.doihttp://dx.doi.org/10.25646/1280
local.edoc.container-titleAntiviral Research
local.edoc.container-textAchazi, K., Patel, P., Paliwal, R., Radonić, A., Niedrig, M., Donoso-Mantke, O. RNA interference inhibits replication of tick-borne encephalitis virus in vitro (2012) Antiviral Research, 93 (1), pp. 94-100.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S0166354211004864
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume93
local.edoc.container-issue1
local.edoc.container-year2012

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