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2013-06-12Zeitschriftenartikel DOI: 10.1371/journal.pone.0066507
Antibody Responses in Humans Infected with Newly Emerging Strains of West Nile Virus in Europe
dc.contributor.authorChabierski, Stefan
dc.contributor.authorMakert, Gustavo R.
dc.contributor.authorKerzhner, Alexandra
dc.contributor.authorBarzon, Luisa
dc.contributor.authorFiebig, Petra
dc.contributor.authorLiebert, Uwe G.
dc.contributor.authorPapa, Anna
dc.contributor.authorRichner, Justin M.
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorDiamond, Michael S.
dc.contributor.authorPalu, Giorgio
dc.contributor.authorUlbert, Sebastian
dc.date.accessioned2018-05-07T16:51:50Z
dc.date.available2018-05-07T16:51:50Z
dc.date.created2013-07-24
dc.date.issued2013-06-12none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rek5d3OUhAuHc/PDF/22NVcEUgBqbY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1613
dc.description.abstractInfection with West Nile Virus (WNV) affects an increasing number of countries worldwide. Although most human infections result in no or mild flu-like symptoms, the elderly and those with a weakened immune system are at higher risk for developing severe neurological disease. Since its introduction into North America in 1999, WNV has spread across the continental United States and caused annual outbreaks with a total of 36,000 documented clinical cases and ~1,500 deaths. In recent years, outbreaks of neuroinvasive disease also have been reported in Europe. The WNV strains isolated during these outbreaks differ from those in North America, as sequencing has revealed that distinct phylogenetic lineages of WNV concurrently circulate in Europe, which has potential implications for the development of vaccines, therapeutics, and diagnostic tests. Here, we studied the human antibody response to European WNV strains responsible for outbreaks in Italy and Greece in 2010, caused by lineage 1 and 2 strains, respectively. The WNV structural proteins were expressed as a series of overlapping fragments fused to a carrier-protein, and binding of IgG in sera from infected persons was analyzed. The results demonstrate that, although the humoral immune response to WNV in humans is heterogeneous, several dominant peptides are recognized.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectHumanseng
dc.subjectAmino Acid Sequenceeng
dc.subjectMolecular Sequence Dataeng
dc.subjectSpecies Specificityeng
dc.subjectEnzyme-Linked Immunosorbent Assayeng
dc.subjectAntibody Formation/immunologyeng
dc.subjectCommunicable Diseases Emerging/immunologyeng
dc.subjectDisease Outbreaks/historyeng
dc.subjectEpitopes B-Lymphocyte/geneticseng
dc.subjectGreece/epidemiologyeng
dc.subjectHistory 21st Centuryeng
dc.subjectImmunoglobulin G/immunologyeng
dc.subjectItaly/epidemiologyeng
dc.subjectProtein Bindingeng
dc.subjectWest Nile Fever/epidemiologyeng
dc.subjectWest Nile Fever/immunologyeng
dc.subjectWest Nile virus/geneticseng
dc.subjectWest Nile virus/immunologyeng
dc.subject.ddc610 Medizin
dc.titleAntibody Responses in Humans Infected with Newly Emerging Strains of West Nile Virus in Europe
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10032106
dc.identifier.doi10.1371/journal.pone.0066507
dc.identifier.doihttp://dx.doi.org/10.25646/1538
local.edoc.container-titlePLoS ONE
local.edoc.container-textChabierski S, Makert GR, Kerzhner A, Barzon L, Fiebig P, et al. (2013) Antibody Responses in Humans Infected with Newly Emerging Strains of West Nile Virus in Europe. PLoS ONE 8(6): e66507.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0066507;jsessionid=48976F2310EE674742A67187C5F0E0F6
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume8
local.edoc.container-issue6
local.edoc.container-year2013

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