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2013-09-09Zeitschriftenartikel DOI: 10.1371/journal.pone.0073052
Serological Evidence of Asymptomatic Infections during Escherichia coli O104:H4 Outbreak in Germany in 2011
dc.contributor.authorBalabanova, Yanina
dc.contributor.authorKlar, Stefanie
dc.contributor.authorDeleré, Yvonne
dc.contributor.authorWilking, Hendrik
dc.contributor.authorFaber, Mirko
dc.contributor.authorLassen, Sofie Gillesberg
dc.contributor.authorGilsdorf, Andreas
dc.contributor.authorDupke, Susann
dc.contributor.authorNitschke, Martin
dc.contributor.authorSayk, Friedhelm
dc.contributor.authorGrunow, Roland
dc.contributor.authorKrause, Gérard
dc.date.accessioned2018-05-07T17:01:39Z
dc.date.available2018-05-07T17:01:39Z
dc.date.created2013-09-18
dc.date.issued2013-09-09none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reSEb2O1Kkw/PDF/22cuMqdcZqWw.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1666
dc.description.abstractIntroduction: The largest known outbreak caused by a rare hybrid strain of Shiga toxin-producing E.coli (STEC) and enteroaggregative E. coli (EAEC) (E.coli O104:H4) of serotype O104:H4 occurred in Germany in 2011. Fenugreek sprouts acted as a transmission vehicle and were widely consumed in the outbreak area at the time of the epidemic. In total 3,842 people developed a clinical illness caused by this strain; however the rates of asymptomatic infections remain unclear. We aimed to develop a serological assay for detection of E.coli O104 LPS specific antibodies and to establish the post-outbreak levels of seropositivity among people with documented exposure to contaminated sprouts. Results and Discussion: Developed serological assays (ELISA with 84% sensitivity, 63% specificity and Western Blot with 100% sensitivity, 82.5% specificity) identified 33% (16/49) level of asymptomatic infection. Relatively small sample size and a significant time- lapse between the onset of symptoms and serum samples collection (appr. 8 weeks) might explain the assay variability. No association was found between clinical or demographic characteristics and assay positivity. Larger studies are needed to understand the complexity of human immune response and factors influencing development of clinical symptoms. Development of intra-outbreak research plans will substantially aid the conduct of more thorough scientific investigation during an outbreak period.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionsepidemiologie
dc.subjectHumanseng
dc.subjectFemaleeng
dc.subjectMaleeng
dc.subjectReproducibility of Resultseng
dc.subjectAgedeng
dc.subjectGermany/epidemiologyeng
dc.subjectMiddle Agedeng
dc.subjectDisease Outbreakseng
dc.subjectEscherichia coli Infections/diagnosiseng
dc.subjectEscherichia coli Infections/epidemiologyeng
dc.subjectAsymptomatic Infections/epidemiologyeng
dc.subjectEscherichia coli/classificationeng
dc.subjectSerotyping/methodseng
dc.subjectShiga-Toxigenic Escherichia coli/classificationeng
dc.subject.ddc610 Medizin
dc.titleSerological Evidence of Asymptomatic Infections during Escherichia coli O104:H4 Outbreak in Germany in 2011
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10032821
dc.identifier.doi10.1371/journal.pone.0073052
dc.identifier.doihttp://dx.doi.org/10.25646/1591
local.edoc.container-titlePLoS ONE
local.edoc.container-textBalabanova Y, Klar S, Deleré Y, Wilking H, Faber MS, et al. (2013) Serological Evidence of Asymptomatic Infections during Escherichia coli O104:H4 Outbreak in Germany in 2011. PLoS ONE 8(9): e73052.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0073052
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume8
local.edoc.container-issue9
local.edoc.container-year2013

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