Discrete and overlapping functions of peptidoglycan synthases in growth, cell division and virulence of Listeria monocytogenes
dc.contributor.author | Rismondo, Jeanine | |
dc.contributor.author | Möller, Lars | |
dc.contributor.author | Aldridge, Christine | |
dc.contributor.author | Gray, Joe | |
dc.contributor.author | Vollmer, Waldemar | |
dc.contributor.author | Halbedel, Sven | |
dc.date.accessioned | 2018-05-07T18:05:35Z | |
dc.date.available | 2018-05-07T18:05:35Z | |
dc.date.created | 2015-01-28 | |
dc.date.issued | 2014-12-19 | none |
dc.identifier.other | http://edoc.rki.de/oa/articles/ret5ybVZcMdww/PDF/207VYe5IyXjI.pdf | |
dc.identifier.uri | http://edoc.rki.de/176904/2013 | |
dc.description.abstract | Upon ingestion of contaminated food, Listeria monocytogenes can cause serious infections in humans that are normally treated with β-lactam antibiotics. These target Listeria's five high molecular weight penicillin-binding proteins (HMW PBPs), which are required for peptidoglycan biosynthesis. The two bi-functional class A HMW PBPs PBP A1 and PBP A2 have transglycosylase and transpeptidase domains catalyzing glycan chain polymerization and peptide cross-linking, respectively, whereas the three class B HMW PBPs B1, B2 and B3 are monofunctional transpeptidases. The precise roles of these PBPs in the cell cycle are unknown. Here we show that green fluorescent protein (GFP)-PBP fusions localized either at the septum, the lateral wall or both, suggesting distinct and overlapping functions. Genetic data confirmed this view: PBP A1 and PBP A2 could not be inactivated simultaneously, and a conditional double mutant strain is largely inducer dependent. PBP B1 is required for rod-shape and PBP B2 for cross-wall biosynthesis and viability, whereas PBP B3 is dispensable for growth and cell division. PBP B1 depletion dramatically increased β-lactam susceptibilities and stimulated spontaneous autolysis but had no effect on peptidoglycan cross-linkage. Our in vitro virulence assays indicated that the complete set of all HMW PBPs is required for maximal virulence. | eng |
dc.language.iso | eng | |
dc.publisher | Robert Koch-Institut, Infektionskrankheiten / Erreger; Robert Koch-Institut, Biologische Sicherheit | |
dc.subject | Animals | eng |
dc.subject | Humans | eng |
dc.subject | Mice | eng |
dc.subject | Bacterial Proteins/metabolism | eng |
dc.subject | Mutation | eng |
dc.subject | Virulence/genetics | eng |
dc.subject | Anti-Bacterial Agents/pharmacology | eng |
dc.subject | Microbial Sensitivity Tests | eng |
dc.subject | HeLa Cells | eng |
dc.subject | 3T3 Cells | eng |
dc.subject | Cell Wall/chemistry | eng |
dc.subject | Cell Wall/physiology | eng |
dc.subject | Listeria monocytogenes/cytology | eng |
dc.subject | Listeria monocytogenes/drug effects | eng |
dc.subject | Listeria monocytogenes/pathogenicity | eng |
dc.subject | Listeria monocytogenes/physiology | eng |
dc.subject | Penicillin-Binding Proteins/genetics | eng |
dc.subject | Penicillin-Binding Proteins/metabolism | eng |
dc.subject | Peptidoglycan/metabolism | eng |
dc.subject | beta-Lactams/pharmacology | eng |
dc.subject.ddc | 610 Medizin | |
dc.title | Discrete and overlapping functions of peptidoglycan synthases in growth, cell division and virulence of Listeria monocytogenes | |
dc.type | periodicalPart | |
dc.identifier.urn | urn:nbn:de:0257-10038604 | |
dc.identifier.doi | 10.1111/mmi.12873 | |
dc.identifier.doi | http://dx.doi.org/10.25646/1938 | |
local.edoc.container-title | Molecular Microbiology | |
local.edoc.container-text | Rismondo, J., Möller, L., Aldridge, C., Gray, J., Vollmer, W., Halbedel, S. Discrete and overlapping functions of peptidoglycan synthases in growth, cell division and virulence of Listeria monocytogenes (2015) Molecular Microbiology, 95 (2), pp. 332-351. | |
local.edoc.fp-subtype | Artikel | |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
local.edoc.container-url | http://onlinelibrary.wiley.com/doi/10.1111/mmi.12873/ | |
local.edoc.container-publisher-name | Wiley | |
local.edoc.container-volume | 95 | |
local.edoc.container-issue | 2 | |
local.edoc.container-year | 2015 |