2015-06-01Zeitschriftenartikel DOI: 10.1038/srep10734
Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
Graewert, Melissa A.
Jeffries, Cy M.
Blanchet, Clement E.
Svergun, Dmitri I.
Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
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