Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
dc.contributor.author | Graewert, Melissa A. | |
dc.contributor.author | Franke, Daniel | |
dc.contributor.author | Jeffries, Cy M. | |
dc.contributor.author | Blanchet, Clement E. | |
dc.contributor.author | Ruskule, Darja | |
dc.contributor.author | Kuhle, Katja | |
dc.contributor.author | Flieger, Antje | |
dc.contributor.author | Schäfer, Bernd | |
dc.contributor.author | Tartsch, Bernd | |
dc.contributor.author | Meijers, Rob | |
dc.contributor.author | Svergun, Dmitri I. | |
dc.date.accessioned | 2018-05-07T18:19:02Z | |
dc.date.available | 2018-05-07T18:19:02Z | |
dc.date.created | 2015-07-03 | |
dc.date.issued | 2015-06-01 | none |
dc.identifier.other | http://edoc.rki.de/oa/articles/rebX7yQ2JYZgk/PDF/21VCShvaY6MOI.pdf | |
dc.identifier.uri | http://edoc.rki.de/176904/2085 | |
dc.description.abstract | Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg). | eng |
dc.language.iso | eng | |
dc.publisher | Robert Koch-Institut, Infektionskrankheiten / Erreger | |
dc.subject | Scattering Small Angle | eng |
dc.subject | Biophysics/methods | eng |
dc.subject | Macromolecular Substances/chemistry | eng |
dc.subject | Macromolecular Substances/isolation & purification | eng |
dc.subject | Solutions/chemistry | eng |
dc.subject.ddc | 610 Medizin | |
dc.title | Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions | |
dc.type | periodicalPart | |
dc.identifier.urn | urn:nbn:de:0257-10039851 | |
dc.identifier.doi | 10.1038/srep10734 | |
dc.identifier.doi | http://dx.doi.org/10.25646/2010 | |
local.edoc.container-title | Scientific Reports | |
local.edoc.fp-subtype | Artikel | |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
local.edoc.container-url | http://www.nature.com/srep/2015/150601/srep10734/full/srep10734.html | |
local.edoc.container-publisher-name | Nature Publishing Group | |
local.edoc.container-volume | 5 | |
local.edoc.container-issue | 10734 | |
local.edoc.container-year | 2015 |