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2015-10-07Zeitschriftenartikel DOI: 10.1371/journal.pone.0139811
Tracing the Spread of Clostridium difficile Ribotype 027 in Germany Based on Bacterial Genome Sequences
dc.contributor.authorSteglich, Matthias
dc.contributor.authorNitsche, Andreas
dc.contributor.authorMüller, Lutz von
dc.contributor.authorHerrmann, Mathias
dc.contributor.authorKohl, Thomas A.
dc.contributor.authorNiemann, Stefan
dc.contributor.authorNübel, Ulrich
dc.date.accessioned2018-05-07T18:42:26Z
dc.date.available2018-05-07T18:42:26Z
dc.date.created2015-12-21
dc.date.issued2015-10-07none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reQY9ERiTAnuw/PDF/29LDo9yfaMqk.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2211
dc.description.abstractWe applied whole-genome sequencing to reconstruct the spatial and temporal dynamics underpinning the expansion of Clostridium difficile ribotype 027 in Germany. Based on re-sequencing of genomes from 57 clinical C. difficile isolates, which had been collected from hospitalized patients at 36 locations throughout Germany between 1990 and 2012, we demonstrate that C. difficile genomes have accumulated sequence variation sufficiently fast to document the pathogen's spread at a regional scale. We detected both previously described lineages of fluoroquinolone-resistant C. difficile ribotype 027, FQR1 and FQR2. Using Bayesian phylogeographic analyses, we show that fluoroquinolone-resistant C. difficile 027 was imported into Germany at least four times, that it had been widely disseminated across multiple federal states even before the first outbreak was noted in 2007, and that it has continued to spread since.eng
dc.language.isoger
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectGermanyeng
dc.subjectHumanseng
dc.subjectBayes Theoremeng
dc.subjectBacterial Proteins/geneticseng
dc.subjectMicrobial Sensitivity Tests/methodseng
dc.subjectHospitalizationeng
dc.subjectClostridium difficile/isolation & purificationeng
dc.subjectEnterocolitis Pseudomembranous/microbiologyeng
dc.subjectAnti-Bacterial Agents/therapeutic useeng
dc.subjectClostridium difficile/drug effectseng
dc.subjectClostridium difficile/geneticseng
dc.subjectEnterocolitis Pseudomembranous/drug therapyeng
dc.subjectFluoroquinolones/therapeutic useeng
dc.subjectGenetic Variation/geneticseng
dc.subjectGenome Bacterial/geneticseng
dc.subjectPhylogeography/methodseng
dc.subjectRibotyping/methodseng
dc.subject.ddc610 Medizin
dc.titleTracing the Spread of Clostridium difficile Ribotype 027 in Germany Based on Bacterial Genome Sequences
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10042330
dc.identifier.doi10.1371/journal.pone.0139811
dc.identifier.doihttp://dx.doi.org/10.25646/2136
local.edoc.container-titlePLoS ONE
local.edoc.container-textSteglich M, Nitsche A, von Müller L, Herrmann M, Kohl TA, Niemann S, et al. (2015) Tracing the Spread of Clostridium difficile Ribotype 027 in Germany Based on Bacterial Genome Sequences. PLoS ONE 10(10): e0139811.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139811
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume10
local.edoc.container-issue10
local.edoc.container-year2015

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