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2009-01-08Zeitschriftenartikel DOI: 10.1186/1471-2180-9-6
Typing Clostridium difficile strains based on tandem repeat sequences
dc.contributor.authorZaiß, Henning
dc.contributor.authorRupnik, Maja
dc.contributor.authorKuijper, Ed J.
dc.contributor.authorHarmanus, Celine
dc.contributor.authorMichielsen, Dolf
dc.contributor.authorJanssens, Koen
dc.contributor.authorNübel, Ulrich
dc.date.accessioned2018-05-07T13:22:41Z
dc.date.available2018-05-07T13:22:41Z
dc.date.created2009-11-09
dc.date.issued2009-01-08none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/regN22ZePO96/PDF/2117SOdhHDtxk.pdf
dc.identifier.urihttp://edoc.rki.de/176904/477
dc.description.abstractBackground: Genotyping of epidemic Clostridium difficile strains is necessary to track their emergence and spread. Portability of genotyping data is desirable to facilitate inter-laboratory comparisons and epidemiological studies. Results: This report presents results from a systematic screen for variation in repetitive DNA in the genome of C. difficile. We describe two tandem repeat loci, designated 'TR6' and 'TR10', which display extensive sequence variation that may be useful for sequence-based strain typing. Based on an investigation of 154 C. difficile isolates comprising 75 ribotypes, tandem repeat sequencing demonstrated excellent concordance with widely used PCR ribotyping and equal discriminatory power. Moreover, tandem repeat sequences enabled the reconstruction of the isolates' largely clonal population structure and evolutionary history. Conclusion: We conclude that sequence analysis of the two repetitive loci introduced here may be highly useful for routine typing of C. difficile. Tandem repeat sequence typing resolves phylogenetic diversity to a level equivalent to PCR ribotypes. DNA sequences may be stored in databases accessible over the internet, obviating the need for the exchange of reference strains.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectHumanseng
dc.subjectMoleculareng
dc.subjectPhylogenyeng
dc.subjectClostridium difficile/classificationeng
dc.subjectClostridium difficile/geneticseng
dc.subjectClostridium difficile/isolation & purificationeng
dc.subjectEnterocolitiseng
dc.subjectRibotypingeng
dc.subjectPseudomembranous/microbiologyeng
dc.subjectEvolutioneng
dc.subjectBacterial Typing Techniques/methodseng
dc.subjectTandem Repeat Sequenceseng
dc.subject.ddc610 Medizin
dc.titleTyping Clostridium difficile strains based on tandem repeat sequences
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-1002823
dc.identifier.doi10.1186/1471-2180-9-6
dc.identifier.doihttp://dx.doi.org/10.25646/402
local.edoc.container-titleBMC Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.biomedcentral.com/1471-2180/9/6
local.edoc.container-publisher-nameBioMed Central
local.edoc.container-volume9
local.edoc.container-issue6
local.edoc.container-year2009

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