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2019-08-26Zeitschriftenartikel DOI: 10.25646/6544
Metaproteomics analysis of microbial diversity of human saliva and tongue dorsum in young healthy individuals
dc.contributor.authorRabe, Alexander
dc.contributor.authorSalazar, Manuela Gesell
dc.contributor.authorMichalik, Stephan
dc.contributor.authorFuchs, Stephan
dc.contributor.authorWelk, Alexander
dc.contributor.authorKocher, Thomas
dc.contributor.authorVölker, Uwe
dc.date.accessioned2020-03-17T07:45:55Z
dc.date.available2020-03-17T07:45:55Z
dc.date.issued2019-08-26none
dc.identifier.other10.1080/20002297.2019.1654786
dc.identifier.urihttp://edoc.rki.de/176904/6513
dc.description.abstractBackground: The human oral microbiome influences initiation or progression of diseases like caries or periodontitis. Metaproteomics approaches enable the simultaneous investigation of microbial and host proteins and their interactions to improve understanding of oral diseases. Objective: In this study, we provide a detailed metaproteomics perspective of the composition of salivary and tongue microbial communities of young healthy subjects. Design: Stimulated saliva and tongue samples were collected from 24 healthy volunteers, subjected to shotgun nLC-MS/MS and analyzed by the Trans-Proteomic Pipeline and the Prophane tool. Results: 3,969 bacterial and 1,857 human proteins could be identified from saliva and tongue, respectively. In total, 1,971 bacterial metaproteins and 1,154 human proteins were shared in both sample types. Twice the amount of bacterial metaproteins were uniquely identified for the tongue dorsum compared to saliva. Overall, 107 bacterial genera of seven phyla formed the microbiome. Comparative analysis identified significant functional differences between the microbial biofilm on the tongue and the microbiome of saliva. Conclusion: Even if the microbial communities of saliva and tongue dorsum showed a strong similarity based on identified protein functions and deduced bacterial composition, certain specific characteristics were observed. Both microbiomes exhibit a great diversity with seven genera being most abundant.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectSalivaeng
dc.subjecttongueeng
dc.subjectmetaproteomicseng
dc.subjecthealthy human oral microbiomeeng
dc.subjectnLC-MS/MSeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleMetaproteomics analysis of microbial diversity of human saliva and tongue dorsum in young healthy individualsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6513-0
dc.identifier.doihttp://dx.doi.org/10.25646/6544
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Oral Microbiologynone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.tandfonline.com/doi/full/10.1080/20002297.2019.1654786?scroll=top&needAccess=truenone
local.edoc.container-publisher-nameTaylor & Francisnone
local.edoc.container-volume11none
local.edoc.container-issue1none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage14none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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